Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 858844..859464 Replicon chromosome
Accession NZ_CP044097
Organism Citrobacter koseri strain FDAARGOS_646

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FOB54_RS04030 Protein ID WP_002892050.1
Coordinates 858844..859062 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag FOB54_RS04035 Protein ID WP_012133514.1
Coordinates 859090..859464 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB54_RS03995 854182..854796 + 615 WP_101743908.1 hypothetical protein -
FOB54_RS04000 854876..855229 + 354 WP_012133521.1 DUF1428 family protein -
FOB54_RS04005 855269..856819 - 1551 WP_114847716.1 EAL domain-containing protein -
FOB54_RS04010 857045..857305 + 261 WP_012133519.1 type B 50S ribosomal protein L31 -
FOB54_RS04015 857307..857447 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
FOB54_RS04020 857525..857995 - 471 WP_114847774.1 hypothetical protein -
FOB54_RS04025 858109..858660 - 552 WP_049009805.1 maltose O-acetyltransferase -
FOB54_RS04030 858844..859062 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FOB54_RS04035 859090..859464 - 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
FOB54_RS04040 859966..863112 - 3147 WP_012133513.1 efflux RND transporter permease AcrB -
FOB54_RS04045 863135..864328 - 1194 WP_012133512.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140043 WP_002892050.1 NZ_CP044097:c859062-858844 [Citrobacter koseri]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T140043 NZ_CP058879:c3600822-3600520 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT140043 WP_012133514.1 NZ_CP044097:c859464-859090 [Citrobacter koseri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT140043 NZ_CP058879:c3600484-3600143 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References