Detailed information of TA system TA140030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 3470292..3470953
• Replicon: chromosome
• Accession: NZ_CP044089
• Organism: Bordetella bronchiseptica strain FDAARGOS_634

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C6NZK6
• Locus tag: FOB42_RS16340
• Protein ID: WP_003817075.1
• Coordinates: 3470292..3470717 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FOB42_RS16345
• Protein ID: WP_003812071.1
• Coordinates: 3470714..3470953 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB42_RS16320	3466100..3467077	+	978	WP_003817071.1	tripartite tricarboxylate transporter substrate binding protein	-
FOB42_RS16325	3467097..3467609	-	513	WP_033461205.1	hypothetical protein	-
FOB42_RS16330	3468108..3469613	+	1506	WP_003812078.1	tripartite tricarboxylate transporter permease	-
FOB42_RS16335	3469718..3470182	-	465	WP_003812075.1	MarR family transcriptional regulator	-
FOB42_RS16340	3470292..3470717	-	426	WP_003817075.1	PIN domain-containing protein	Toxin
FOB42_RS16345	3470714..3470953	-	240	WP_003812071.1	Arc family DNA-binding protein	Antitoxin
FOB42_RS16350	3471027..3471869	-	843	WP_033448622.1	AraC family transcriptional regulator	-
FOB42_RS16355	3472007..3472552	+	546	WP_003817079.1	peroxidase-related enzyme	-
FOB42_RS16360	3472611..3473123	+	513	WP_033448625.1	redoxin domain-containing protein	-
FOB42_RS16365	3473204..3473437	+	234	WP_033448627.1	DUF2945 domain-containing protein	-
FOB42_RS16370	3473434..3473994	+	561	WP_033448630.1	DUF488 domain-containing protein	-
FOB42_RS16375	3474072..3475118	+	1047	WP_003817082.1	Dyp-type peroxidase	-
FOB42_RS16380	3475115..3475918	+	804	WP_003817085.1	bacteriocin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 14813.19 Da
• Isoelectric Point: 6.3704

>T140030 WP_003817075.1 NZ_CP044089:c3470717-3470292 [Bordetella bronchiseptica]
MILLDTDVLLEPLRPAPDPAVAAWLDAQHVETLYLAAPGAAEIQLGLARLPRGKRAEALRHEFERRILPLFMGHVLPFDA
AAADAYAAVMMRAAAAGHALCATDGCIAAIATAHGLAIATRHPARFEAAGLTAVDPWHAAA

Nucleotide

• Length: 426 bp

>T140030 NZ_CP058879:351714-351989 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8561.78 Da
• Isoelectric Point: 11.5658

>AT140030 WP_003812071.1 NZ_CP044089:c3470953-3470714 [Bordetella bronchiseptica]
VSSITVRNVPEEVHRALRVRAATHGRSTEAEIRAILENAVLPDGRVKLGTLLSHIGRGARGMDLTIERNPAPARPASFE

Nucleotide

• Length: 240 bp

>AT140030 NZ_CP058879:351986-352345 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0C6NZK6