Detailed information of TA system TA140029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3337916..3339515
• Replicon: chromosome
• Accession: NZ_CP044089
• Organism: Bordetella bronchiseptica strain FDAARGOS_634

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: FOB42_RS15700
• Protein ID: WP_003816876.1
• Coordinates: 3338175..3339515 (+)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FOB42_RS15695
• Protein ID: WP_019247974.1
• Coordinates: 3337916..3338173 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB42_RS15675	3333584..3334189	-	606	WP_003816870.1	DUF2239 family protein	-
FOB42_RS15680	3334412..3335872	+	1461	WP_003812368.1	IMP dehydrogenase	-
FOB42_RS15685	3335969..3337561	+	1593	WP_003816871.1	glutamine-hydrolyzing GMP synthase	-
FOB42_RS15695	3337916..3338173	+	258	WP_019247974.1	helix-turn-helix domain-containing protein	Antitoxin
FOB42_RS15700	3338175..3339515	+	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
FOB42_RS15705	3339611..3340300	-	690	WP_033460881.1	TetR/AcrR family transcriptional regulator	-
FOB42_RS15710	3340313..3341743	+	1431	WP_033448398.1	MFS transporter	-
FOB42_RS15715	3341975..3343054	+	1080	WP_003816883.1	tellurite resistance/C4-dicarboxylate transporter family protein	-
FOB42_RS15720	3343470..3344288	+	819	WP_050450637.1	IclR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T140029 WP_003816876.1 NZ_CP044089:3338175-3339515 [Bordetella bronchiseptica]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T140029 NZ_CP058879:267605-267712 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT140029 WP_019247974.1 NZ_CP044089:3337916-3338173 [Bordetella bronchiseptica]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT140029 NZ_CP058879:c267556-267493 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATAACTGCAACGTGCGGGGGTTTT

Structures

Toxin

• AlphaFold DB: Q7VVM6