Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4401491..4402122 Replicon chromosome
Accession NZ_CP044083
Organism Klebsiella aerogenes strain FDAARGOS_641

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H3FL31
Locus tag FOB49_RS21385 Protein ID WP_015703741.1
Coordinates 4401491..4401766 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0H3FNK6
Locus tag FOB49_RS21390 Protein ID WP_015703740.1
Coordinates 4401763..4402122 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB49_RS21365 4396720..4397055 + 336 WP_015703743.1 universal stress protein UspB -
FOB49_RS21370 4397120..4398616 - 1497 WP_015369272.1 inorganic phosphate transporter PitA -
FOB49_RS21375 4398847..4400040 + 1194 WP_015703742.1 NAD(P)/FAD-dependent oxidoreductase -
FOB49_RS21380 4400081..4401094 - 1014 WP_015369274.1 magnesium transporter -
FOB49_RS21385 4401491..4401766 + 276 WP_015703741.1 type II toxin-antitoxin system HicA family toxin Toxin
FOB49_RS21390 4401763..4402122 + 360 WP_015703740.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FOB49_RS21395 4402150..4402551 - 402 WP_015703739.1 nickel-responsive transcriptional regulator NikR -
FOB49_RS21400 4402539..4403330 - 792 WP_015703738.1 nickel import ATP-binding protein NikE -
FOB49_RS21405 4403327..4404091 - 765 WP_015703737.1 nickel import ATP-binding protein NikD -
FOB49_RS21410 4404091..4404924 - 834 WP_015703736.1 nickel ABC transporter permease subunit NikC -
FOB49_RS21415 4404921..4405865 - 945 WP_015703735.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10258.89 Da        Isoelectric Point: 10.4888

>T140023 WP_015703741.1 NZ_CP044083:4401491-4401766 [Klebsiella aerogenes]
MEQQVLSLRKKQRNTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T140023 NZ_CP058875:141531-141818 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13305.02 Da        Isoelectric Point: 4.3472

>AT140023 WP_015703740.1 NZ_CP044083:4401763-4402122 [Klebsiella aerogenes]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCREAGIEPYAQPEKLKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT140023 NZ_CP058875:141283-141534 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FL31

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FNK6

References