Detailed information of TA system TA140008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1422669..1423243
• Replicon: chromosome
• Accession: NZ_CP044077
• Organism: Pasteurella multocida strain FDAARGOS_644

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FOB52_RS06800
• Protein ID: WP_061406134.1
• Coordinates: 1422669..1422953 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FOB52_RS06805
• Protein ID: WP_150332091.1
• Coordinates: 1422950..1423243 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB52_RS06780	1418435..1420051	-	1617	WP_150332090.1	AAA family ATPase	-
FOB52_RS06785	1420336..1420971	-	636	Protein_1294	NERD domain-containing protein	-
FOB52_RS06790	1421031..1421390	+	360	WP_095261631.1	DUF3418 domain-containing protein	-
FOB52_RS06800	1422669..1422953	+	285	WP_061406134.1	hypothetical protein	Toxin
FOB52_RS06805	1422950..1423243	+	294	WP_150332091.1	putative addiction module antidote protein	Antitoxin
FOB52_RS06810	1423646..1424323	-	678	WP_005751574.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
FOB52_RS06815	1424342..1424809	-	468	WP_005726755.1	PTS sugar transporter subunit IIA	-
FOB52_RS06820	1424854..1426644	-	1791	WP_005726754.1	PTS ascorbate-specific subunit IIBC	-
FOB52_RS06825	1426994..1428085	+	1092	WP_005751571.1	L-ascorbate 6-phosphate lactonase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-87)

Antitoxin

• Uncharacterized protein HI1420 (7-92)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10667.40 Da
• Isoelectric Point: 10.2297

>T140008 WP_061406134.1 NZ_CP044077:1422669-1422953 [Pasteurella multocida]
VGRLKEVKILSAKSAVLARINKAMNGNFGDHKSIGNGLYEMRIIKGSGYRVYYGQYREVTYLLICGGDKSTQKSDIVKAR
ELWKEIKQQEEVKV

Nucleotide

• Length: 285 bp

>T140008 NZ_CP058874:2120584-2120686 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.94 Da
• Isoelectric Point: 9.7881

>AT140008 WP_150332091.1 NZ_CP044077:1422950-1423243 [Pasteurella multocida]
MSKKKIKLKLFDIAKHLDSEEMIAAYLSEVLETCDTNEFISALSDVVRAKGMTELAAKTGLRRESLYKTLSYGSKPRFDT
IMKIMHSLGIKLVPTHI

Nucleotide

• Length: 294 bp

>AT140008 NZ_CP058874:c2120724-2120456 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT