Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4195493..4196136 Replicon chromosome
Accession NZ_CP044074
Organism Pseudomonas oryzihabitans strain FDAARGOS_657

Toxin (Protein)


Gene name vapC Uniprot ID A0A1G5NTI6
Locus tag FOB65_RS20890 Protein ID WP_074584223.1
Coordinates 4195723..4196136 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A329HNZ8
Locus tag FOB65_RS20885 Protein ID WP_027597360.1
Coordinates 4195493..4195723 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB65_RS20870 4190883..4192742 + 1860 WP_190971329.1 GGDEF domain-containing protein -
FOB65_RS20875 4192739..4193455 + 717 WP_150340421.1 transporter substrate-binding domain-containing protein -
FOB65_RS20880 4193474..4195198 - 1725 WP_042134704.1 ubiquinone-dependent pyruvate dehydrogenase -
FOB65_RS20885 4195493..4195723 + 231 WP_027597360.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FOB65_RS20890 4195723..4196136 + 414 WP_074584223.1 tRNA(fMet)-specific endonuclease VapC Toxin
FOB65_RS20895 4196434..4196802 + 369 WP_007159937.1 glycine cleavage system protein GcvH -
FOB65_RS20900 4196810..4199665 + 2856 WP_150340423.1 aminomethyl-transferring glycine dehydrogenase -
FOB65_RS20905 4199878..4200987 + 1110 WP_074584221.1 glycine cleavage system aminomethyltransferase GcvT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15132.33 Da        Isoelectric Point: 7.8849

>T140004 WP_074584223.1 NZ_CP044074:4195723-4196136 [Pseudomonas oryzihabitans]
MLKFMLDTNTCIFTIKNRPTSVREAFTRHQGQLCISTITLMELIYGAEKSSSPERNLAVIEGFAARLEVLTYGPEAAAHT
GQLRAELARAGQQIGPYDQMIAGHARSLGLIVVTNNRREFDRVPGLRVEDWVTAAGN

Download         Length: 414 bp

>T140004 NZ_CP058874:381956-382333 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8378.32 Da        Isoelectric Point: 4.3867

>AT140004 WP_027597360.1 NZ_CP044074:4195493-4195723 [Pseudomonas oryzihabitans]
MDQGAVFQSNRSQAVRLPKAVALPADVKRVDVVAVGRTRIITPAGESWDSWFEGEGVTADFMAERDQPAEQEREGF

Download         Length: 231 bp

>AT140004 NZ_CP058874:381738-381959 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G5NTI6

Antitoxin

Source ID Structure
AlphaFold DB A0A329HNZ8

References