Detailed information of TA system TA140001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 113966..114603
• Replicon: plasmid unnamed1
• Accession: NZ_CP044072
• Organism: Pseudomonas oryzihabitans strain FDAARGOS_657

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: FOB65_RS00565
• Protein ID: WP_190971308.1
• Coordinates: 113966..114364 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: FOB65_RS00570
• Protein ID: WP_150338108.1
• Coordinates: 114373..114603 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB65_RS00540	109391..109816	+	426	WP_150338104.1	VOC family protein	-
FOB65_RS00545	109913..110674	+	762	Protein_103	5-oxoprolinase subunit PxpB	-
FOB65_RS00550	110671..111612	+	942	WP_150338105.1	biotin-dependent carboxyltransferase family protein	-
FOB65_RS00555	111701..113020	+	1320	WP_150338106.1	MFS transporter	-
FOB65_RS00560	113080..113772	+	693	WP_150338132.1	DUF1445 domain-containing protein	-
FOB65_RS00565	113966..114364	-	399	WP_190971308.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOB65_RS00570	114373..114603	-	231	WP_150338108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FOB65_RS00575	114841..115122	+	282	WP_150338109.1	hypothetical protein	-
FOB65_RS00580	115530..118007	+	2478	WP_150338110.1	hypothetical protein	-
FOB65_RS00585	118419..119075	-	657	WP_150338111.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..132067	132067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14605.97 Da
• Isoelectric Point: 7.7788

>T140001 WP_190971308.1 NZ_CP044072:c114364-113966 [Pseudomonas oryzihabitans]
MLDTCICSFIMREHPAAVIRRLSAEVERGNRIVISAITYAEMRYGQIGKKASPKHKVLVDEFVRRLDAVIAWDLRAVDAT
VEVMRQLNAAGTPIGPNDTAIAGHAIAIGCTLVTNNVREFSRIPGLVYEDWV

Nucleotide

• Length: 399 bp

>T140001 NZ_CP058874:274121-274228 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8438.55 Da
• Isoelectric Point: 4.5045

>AT140001 WP_150338108.1 NZ_CP044072:c114603-114373 [Pseudomonas oryzihabitans]
MRTVSIFMNGRNQAVRLPQDMAFDGIGELEISKEGDVITLRPARPSWTSLAELPKADADFLQERTAVASDEGRFTL

Nucleotide

• Length: 231 bp

>AT140001 NZ_CP058874:c274072-274006 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC