Detailed information of TA system TA139965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 2737140..2737840
• Replicon: chromosome
• Accession: NZ_CP044058
• Organism: Burkholderia multivorans strain FDAARGOS_622

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: FOB30_RS27025
• Protein ID: WP_053296986.1
• Coordinates: 2737140..2737517 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: B9BQB8
• Locus tag: FOB30_RS27030
• Protein ID: WP_006405635.1
• Coordinates: 2737517..2737840 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB30_RS27000	2732684..2733130	+	447	WP_006405630.1	VOC family protein	-
FOB30_RS27005	2733234..2734784	-	1551	WP_006398698.1	sodium:solute symporter family protein	-
FOB30_RS27010	2734781..2735014	-	234	WP_006398697.1	DUF3311 domain-containing protein	-
FOB30_RS27015	2735211..2736128	-	918	WP_053296988.1	spermidine synthase	-
FOB30_RS27020	2736356..2736610	+	255	WP_053296987.1	hypothetical protein	-
FOB30_RS31375	2736787..2737155	-	369	WP_190972330.1	hypothetical protein	-
FOB30_RS27025	2737140..2737517	+	378	WP_053296986.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOB30_RS27030	2737517..2737840	+	324	WP_006405635.1	helix-turn-helix domain-containing protein	Antitoxin
FOB30_RS27035	2737863..2738354	-	492	WP_053296985.1	DNA-deoxyinosine glycosylase	-
FOB30_RS27040	2738351..2738965	-	615	WP_006398690.1	chorismate lyase	-
FOB30_RS27045	2738974..2740872	-	1899	WP_006409758.1	molecular chaperone HtpG	-
FOB30_RS27055	2741031..2742491	-	1461	WP_150331814.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13480.42 Da
• Isoelectric Point: 8.9407

>T139965 WP_053296986.1 NZ_CP044058:2737140-2737517 [Burkholderia multivorans]
MGINSSMVPYTKPLYWLGSARKDLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGFGSAGVLEVVESEDSGTYRAVYTV
KLGGAVYVLHCFQKKSCRGIATPKPDIELVRARLSAAEAHANGET

Nucleotide

• Length: 378 bp

>T139965 NZ_CP058859:3947231-3947449 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11828.69 Da
• Isoelectric Point: 8.0686

>AT139965 WP_006405635.1 NZ_CP044058:2737517-2737840 [Burkholderia multivorans]
MIEIERGSGNVYADLGTPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSETEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT139965 NZ_CP058859:3946830-3947204 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: B9BQB8