Detailed information of TA system TA139962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 991449..992719
• Replicon: chromosome
• Accession: NZ_CP044056
• Organism: Burkholderia multivorans strain FDAARGOS_622

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOB30_RS04530
• Protein ID: WP_053298292.1
• Coordinates: 992141..992719 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FOB30_RS04525
• Protein ID: WP_053298293.1
• Coordinates: 991449..992006 (+)
• Length: 186 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB30_RS04495	986745..987770	+	1026	WP_006406131.1	aldo/keto reductase	-
FOB30_RS04500	987981..988313	+	333	WP_006406130.1	antibiotic biosynthesis monooxygenase	-
FOB30_RS04505	988487..988864	-	378	WP_150331542.1	hypothetical protein	-
FOB30_RS04510	988902..989522	+	621	WP_053298295.1	cysteine hydrolase	-
FOB30_RS04515	989533..990936	-	1404	WP_053298294.1	hypothetical protein	-
FOB30_RS04525	991449..992006	+	558	WP_053298293.1	helix-turn-helix domain-containing protein	Antitoxin
FOB30_RS04530	992141..992719	+	579	WP_053298292.1	HD domain-containing protein	Toxin
FOB30_RS04535	992820..994358	-	1539	WP_053298291.1	MFS transporter	-
FOB30_RS04540	994537..995457	+	921	WP_006406123.1	LysR family transcriptional regulator	-
FOB30_RS04545	995504..996643	-	1140	WP_053298290.1	enoyl-CoA hydratase/isomerase family protein	-
FOB30_RS04550	996640..997431	-	792	WP_053298289.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-173)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21358.07 Da
• Isoelectric Point: 4.9434

>T139962 WP_053298292.1 NZ_CP044056:992141-992719 [Burkholderia multivorans]
MNPDTIRARLDFLREAERLKDVLRSGRTSSGRREGTAEHSWRLCLMALVFADALPGIDTLKLLKLCVVHDLGEALHGDIP
ATEQAAHPDKNMHERDDLLTLTAALDPVLRNELVALWDEYDAAASPEARAAKALDKLETILQHTQGANPPDFDYAFNLDY
GRRYTDAAPLFSAIRAIVDADTQRRIDAQAQT

Nucleotide

• Length: 579 bp

>T139962 NZ_CP058859:1820024-1820126 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 186 a.a.
• Molecular weight: 20383.32 Da
• Isoelectric Point: 8.1892

>AT139962 WP_053298293.1 NZ_CP044056:991449-992006 [Burkholderia multivorans]
MPDSPLHTPPLGPQLKRWRLLHRVKQSHAAELFGVAQSTISRWEAGVQQMSPDERATAERLLAARLDSAGDHALARLVAG
SAGRMHLVCDLTHRLLACSPSRAAEFSQPLSMLLGTSLWRYATEEIVRMESALDTLGWHDCAGPPSVEFDTGTNASRVVP
IHRSRCRWTRIMLSDGSAARLVETL

Nucleotide

• Length: 558 bp

>AT139962 NZ_CP058859:c1820130-1819983 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG