Detailed information of TA system TA139960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 704061..705808
• Replicon: chromosome
• Accession: NZ_CP044056
• Organism: Burkholderia multivorans strain FDAARGOS_622

Toxin (Protein)

• Gene name: doc
• Uniprot ID: B9BHC0
• Locus tag: FOB30_RS03170
• Protein ID: WP_006403210.1
• Coordinates: 704912..705808 (-)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B9AZJ2
• Locus tag: FOB30_RS03165
• Protein ID: WP_006396780.1
• Coordinates: 704061..704915 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB30_RS03145	699232..700722	-	1491	WP_006403213.1	aldehyde dehydrogenase	-
FOB30_RS03150	700784..701614	-	831	WP_006403212.1	aspartate dehydrogenase	-
FOB30_RS03155	701607..703274	-	1668	WP_053298420.1	thiamine pyrophosphate-binding protein	-
FOB30_RS03160	703267..704064	-	798	WP_053298419.1	SDR family oxidoreductase	-
FOB30_RS03165	704061..704915	-	855	WP_006396780.1	IclR family transcriptional regulator	Antitoxin
FOB30_RS03170	704912..705808	-	897	WP_006403210.1	alpha/beta fold hydrolase	Toxin
FOB30_RS03175	705827..706354	-	528	WP_006396778.1	cupin domain-containing protein	-
FOB30_RS03180	706382..707152	-	771	WP_059940455.1	SDR family oxidoreductase	-
FOB30_RS03185	707145..708215	-	1071	WP_006403208.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
FOB30_RS03190	708263..708574	-	312	WP_053298417.1	hypothetical protein	-
FOB30_RS03195	708576..708893	-	318	WP_053298416.1	non-heme iron oxygenase ferredoxin subunit	-
FOB30_RS03200	709329..710408	+	1080	WP_039217461.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-280)

Antitoxin

• Transcription regulator IclR, N-terminal (23-71)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30544.80 Da
• Isoelectric Point: 8.0446

>T139960 WP_006403210.1 NZ_CP044056:c705808-704912 [Burkholderia multivorans]
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQQAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAGVGLQVIPQAGHAGYVEAPDVYSALIDAFCRQCDIERRAA

Nucleotide

• Length: 897 bp

>T139960 NZ_CP058859:318539-318907 [Klebsiella quasipneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCGGACTTCGTCGGCATGACCGTCGAGGCGGC
TGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31176.75 Da
• Isoelectric Point: 6.6558

>AT139960 WP_006396780.1 NZ_CP044056:c704915-704061 [Burkholderia multivorans]
MSEPLQSAAEPAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSEAPVFSSIRVNVGTRLPAHATTHGHV
LMGDLSLDELRALYPEGTLPRMTNATPDTVDALYTMIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVPR
PEIDATLIADGLIDKVRRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 855 bp

>AT139960 NZ_CP058859:318321-318542 [Klebsiella quasipneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATAGATG
ATGAGTTCGCGGCAATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: B9BHC0

Antitoxin

• AlphaFold DB: B9AZJ2