Detailed information of TA system TA139958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 265116..266155
• Replicon: chromosome
• Accession: NZ_CP044056
• Organism: Burkholderia multivorans strain FDAARGOS_622

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: FOB30_RS01205
• Protein ID: WP_045593318.1
• Coordinates: 265580..266155 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A8E2S2M5
• Locus tag: FOB30_RS01200
• Protein ID: WP_053298663.1
• Coordinates: 265116..265583 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB30_RS01175	260586..262049	+	1464	WP_006403683.1	5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase	-
FOB30_RS01180	262092..262940	+	849	WP_006402379.1	3,4-dihydroxyphenylacetate 2,3-dioxygenase	-
FOB30_RS01185	262976..263368	+	393	WP_053298610.1	5-carboxymethyl-2-hydroxymuconate Delta-isomerase	-
FOB30_RS01190	263383..264186	+	804	WP_006402377.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
FOB30_RS01195	264194..265000	+	807	WP_053298609.1	4-hydroxy-2-oxoheptanedioate aldolase	-
FOB30_RS01200	265116..265583	+	468	WP_053298663.1	helix-turn-helix domain-containing protein	Antitoxin
FOB30_RS01205	265580..266155	+	576	WP_045593318.1	PIN domain-containing protein	Toxin
FOB30_RS01210	266400..267209	-	810	WP_053298608.1	helix-turn-helix transcriptional regulator	-
FOB30_RS01215	267305..267556	+	252	WP_039218251.1	hypothetical protein	-
FOB30_RS01220	267635..268600	+	966	WP_053298607.1	zinc-binding alcohol dehydrogenase family protein	-
FOB30_RS01225	268659..268814	-	156	WP_081000698.1	porin	-
FOB30_RS01230	269068..269547	+	480	WP_053298662.1	hypothetical protein	-
FOB30_RS01235	269683..270765	-	1083	WP_053298606.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21555.65 Da
• Isoelectric Point: 4.8924

>T139958 WP_045593318.1 NZ_CP044056:265580-266155 [Burkholderia multivorans]
MTHSPFTAIYDACVLYPAPLRDFLMRLALSGCFRARWSAHITDEWKRNLLDNRPDLTAEQLDRTSSLMDRAIPDAVVTGY
ESLIAGLVLPDADDRHVLAAAIRCNASVIVTFNRKDFPEEALLPYGIEAQHPDEFVDDLFDLEPAVVVAAARRQREALKQ
PPLTADRYLEILRRQGMVQTCQSLDAYRALL

Nucleotide

• Length: 576 bp

>T139958 NZ_CP058857:c5380284-5379901 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17669.09 Da
• Isoelectric Point: 5.8709

>AT139958 WP_053298663.1 NZ_CP044056:265116-265583 [Burkholderia multivorans]
MHHATTPDMILPVEREMQAAVEAQRMLAAYLATRTDTQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPYHRTGKHRRVRFSDLMRYKAERDQESADAMEELSKQAQDLRMGYE

Nucleotide

• Length: 468 bp

>AT139958 NZ_CP058857:c5380526-5380284 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA