Detailed information of TA system TA139956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2162110..2162687
• Replicon: chromosome
• Accession: NZ_CP044055
• Organism: Burkholderia multivorans strain FDAARGOS_623

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOB31_RS30165
• Protein ID: WP_053298683.1
• Coordinates: 2162322..2162687 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S9ML45
• Locus tag: FOB31_RS30160
• Protein ID: WP_035956044.1
• Coordinates: 2162110..2162328 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB31_RS30140	2157847..2159118	-	1272	WP_053298681.1	Zn-dependent hydrolase	-
FOB31_RS30145	2159111..2159650	-	540	WP_053298682.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
FOB31_RS30150	2159770..2160936	-	1167	WP_006403832.1	branched-chain amino acid ABC transporter substrate-binding protein	-
FOB31_RS30155	2161112..2162008	+	897	WP_035954582.1	LysR family transcriptional regulator	-
FOB31_RS30160	2162110..2162328	+	219	WP_035956044.1	hypothetical protein	Antitoxin
FOB31_RS30165	2162322..2162687	+	366	WP_053298683.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOB31_RS30170	2162735..2163168	-	434	Protein_1888	GNAT family N-acetyltransferase	-
FOB31_RS30175	2163418..2163981	+	564	WP_006399806.1	peroxiredoxin	-
FOB31_RS30180	2164099..2165697	+	1599	WP_053298685.1	alkyl hydroperoxide reductase subunit F	-
FOB31_RS30185	2165759..2166487	-	729	WP_190972338.1	hypothetical protein	-
FOB31_RS30190	2166611..2167510	-	900	WP_053298686.1	lipid A hydroxylase LpxO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13556.55 Da
• Isoelectric Point: 5.1515

>T139956 WP_053298683.1 NZ_CP044055:2162322-2162687 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDHRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDSTVADRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPSTEPGIRVPYQI

Nucleotide

• Length: 366 bp

>T139956 NZ_CP058857:c4899951-4899667 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.38 Da
• Isoelectric Point: 4.7640

>AT139956 WP_035956044.1 NZ_CP044055:2162110-2162328 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Nucleotide

• Length: 219 bp

>AT139956 NZ_CP058857:c4900183-4899941 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9ML45