Detailed information of TA system TA139955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1987542..1988581
• Replicon: chromosome
• Accession: NZ_CP044055
• Organism: Burkholderia multivorans strain FDAARGOS_623

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: FOB31_RS29390
• Protein ID: WP_045593318.1
• Coordinates: 1987542..1988117 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A8E2S2M5
• Locus tag: FOB31_RS29395
• Protein ID: WP_053298663.1
• Coordinates: 1988114..1988581 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB31_RS29360	1982932..1984014	+	1083	WP_053298606.1	porin	-
FOB31_RS29365	1984150..1984629	-	480	WP_053298662.1	hypothetical protein	-
FOB31_RS29370	1984883..1985038	+	156	WP_081000698.1	porin	-
FOB31_RS29375	1985097..1986062	-	966	WP_053298607.1	zinc-binding alcohol dehydrogenase family protein	-
FOB31_RS29380	1986141..1986392	-	252	WP_039218251.1	hypothetical protein	-
FOB31_RS29385	1986488..1987297	+	810	WP_053298608.1	helix-turn-helix transcriptional regulator	-
FOB31_RS29390	1987542..1988117	-	576	WP_045593318.1	PIN domain-containing protein	Toxin
FOB31_RS29395	1988114..1988581	-	468	WP_053298663.1	helix-turn-helix domain-containing protein	Antitoxin
FOB31_RS29400	1988697..1989503	-	807	WP_053298609.1	4-hydroxy-2-oxoheptanedioate aldolase	-
FOB31_RS29405	1989511..1990314	-	804	WP_006402377.1	2-oxo-hepta-3-ene-1,7-dioic acid hydratase	-
FOB31_RS29410	1990329..1990721	-	393	WP_053298610.1	5-carboxymethyl-2-hydroxymuconate Delta-isomerase	-
FOB31_RS29415	1990757..1991605	-	849	WP_006402379.1	3,4-dihydroxyphenylacetate 2,3-dioxygenase	-
FOB31_RS29420	1991648..1993111	-	1464	WP_006403683.1	5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21555.65 Da
• Isoelectric Point: 4.8924

>T139955 WP_045593318.1 NZ_CP044055:c1988117-1987542 [Burkholderia multivorans]
MTHSPFTAIYDACVLYPAPLRDFLMRLALSGCFRARWSAHITDEWKRNLLDNRPDLTAEQLDRTSSLMDRAIPDAVVTGY
ESLIAGLVLPDADDRHVLAAAIRCNASVIVTFNRKDFPEEALLPYGIEAQHPDEFVDDLFDLEPAVVVAAARRQREALKQ
PPLTADRYLEILRRQGMVQTCQSLDAYRALL

Nucleotide

• Length: 576 bp

>T139955 NZ_CP058857:c4804758-4804381 [Klebsiella pneumoniae]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17669.09 Da
• Isoelectric Point: 5.8709

>AT139955 WP_053298663.1 NZ_CP044055:c1988581-1988114 [Burkholderia multivorans]
MHHATTPDMILPVEREMQAAVEAQRMLAAYLATRTDTQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPYHRTGKHRRVRFSDLMRYKAERDQESADAMEELSKQAQDLRMGYE

Nucleotide

• Length: 468 bp

>AT139955 NZ_CP058857:c4805216-4804848 [Klebsiella pneumoniae]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCATGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA