Detailed information of TA system TA139953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1547889..1549636
• Replicon: chromosome
• Accession: NZ_CP044055
• Organism: Burkholderia multivorans strain FDAARGOS_623

Toxin (Protein)

• Gene name: doc
• Uniprot ID: B9BHC0
• Locus tag: FOB31_RS27425
• Protein ID: WP_006403210.1
• Coordinates: 1547889..1548785 (+)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B9AZJ2
• Locus tag: FOB31_RS27430
• Protein ID: WP_006396780.1
• Coordinates: 1548782..1549636 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB31_RS27395	1543289..1544368	-	1080	WP_039217461.1	porin	-
FOB31_RS27400	1544804..1545121	+	318	WP_053298416.1	non-heme iron oxygenase ferredoxin subunit	-
FOB31_RS27405	1545123..1545434	+	312	WP_053298417.1	hypothetical protein	-
FOB31_RS27410	1545482..1546552	+	1071	WP_006403208.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
FOB31_RS27415	1546545..1547315	+	771	WP_059940455.1	SDR family oxidoreductase	-
FOB31_RS27420	1547343..1547870	+	528	WP_006396778.1	cupin domain-containing protein	-
FOB31_RS27425	1547889..1548785	+	897	WP_006403210.1	alpha/beta fold hydrolase	Toxin
FOB31_RS27430	1548782..1549636	+	855	WP_006396780.1	IclR family transcriptional regulator	Antitoxin
FOB31_RS27435	1549633..1550430	+	798	WP_053298419.1	SDR family oxidoreductase	-
FOB31_RS27440	1550423..1552090	+	1668	WP_053298420.1	thiamine pyrophosphate-binding protein	-
FOB31_RS27445	1552083..1552913	+	831	WP_006403212.1	aspartate dehydrogenase	-
FOB31_RS27450	1552975..1554465	+	1491	WP_006403213.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-280)

Antitoxin

• Transcription regulator IclR, N-terminal (23-71)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30544.80 Da
• Isoelectric Point: 8.0446

>T139953 WP_006403210.1 NZ_CP044055:1547889-1548785 [Burkholderia multivorans]
MSVVDKRERDRTAAFATLLERFPEQRCAAGAAGTIGYREAGAQQAGAALPVVLLHGIGSGAASWVRQLDALGASRRVLAW
DAPGYGASTPVHGASPSAVDYAASLDAWLDALGIARCVLVGHSLGAIVAGGMVRVKPERIAGLLLISPAGGYGSAPAETR
ASRRDARLAMLAELGPAGLAAQRSGNMLSGFATEDARAWVRWNMARIVPAGYAQATHLLANADLAADLAGFRGRTAVAVG
ANDAITPPAACERIAAAAGVGLQVIPQAGHAGYVEAPDVYSALIDAFCRQCDIERRAA

Nucleotide

• Length: 897 bp

>T139953 NZ_CP058857:4159424-4159642 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31176.75 Da
• Isoelectric Point: 6.6558

>AT139953 WP_006396780.1 NZ_CP044055:1548782-1549636 [Burkholderia multivorans]
MSEPLQSAAEPAKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSEAPVFSSIRVNVGTRLPAHATTHGHV
LMGDLSLDELRALYPEGTLPRMTNATPDTVDALYTMIREDALRGYGISNSSFERGISVVTAPVRNDTQKIVACITVTVPR
PEIDATLIADGLIDKVRRAAAELSRRLNYRSDDEHTFLKALGLR

Nucleotide

• Length: 855 bp

>AT139953 NZ_CP058857:4159023-4159397 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: B9BHC0

Antitoxin

• AlphaFold DB: B9AZJ2