Detailed information of TA system TA139950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 217228..217848
• Replicon: chromosome
• Accession: NZ_CP044055
• Organism: Burkholderia multivorans strain FDAARGOS_623

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FOB31_RS21190
• Protein ID: WP_035487939.1
• Coordinates: 217228..217545 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: B9B269
• Locus tag: FOB31_RS21195
• Protein ID: WP_006398012.1
• Coordinates: 217549..217848 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB31_RS21175	213761..215194	-	1434	WP_006404214.1	aldehyde dehydrogenase family protein	-
FOB31_RS21180	215223..216863	-	1641	WP_053297866.1	acetolactate synthase large subunit	-
FOB31_RS21190	217228..217545	+	318	WP_035487939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOB31_RS21195	217549..217848	+	300	WP_006398012.1	putative addiction module antidote protein	Antitoxin
FOB31_RS21200	217883..219169	-	1287	WP_053297867.1	DUF445 domain-containing protein	-
FOB31_RS21205	219284..220504	-	1221	WP_053297868.1	FAD-dependent oxidoreductase	-
FOB31_RS21210	220494..220820	-	327	WP_006404219.1	non-heme iron oxygenase ferredoxin subunit	-
FOB31_RS21215	220838..221326	-	489	WP_006398008.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
FOB31_RS21220	221341..222612	-	1272	WP_053297869.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-89)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12092.91 Da
• Isoelectric Point: 10.2349

>T139950 WP_035487939.1 NZ_CP044055:217228-217545 [Burkholderia multivorans]
MPYLAPTFSIRTTAVFDAWFAGLQDRTAKRRIQARIDRLSMGNLGNWKSVGSPVYEIRIDHGPGYRVYFVRRRTIWVVLL
CGGDKSTQRDDIRAAHAMLEHLDLE

Nucleotide

• Length: 318 bp

>T139950 NZ_CP058857:937355-937765 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10605.00 Da
• Isoelectric Point: 6.6381

>AT139950 WP_006398012.1 NZ_CP044055:217549-217848 [Burkholderia multivorans]
MSKISTRPWDSAEHLKTDDDMAEYFEACLQEGGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT139950 NZ_CP058857:937108-937374 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3KTI5