Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4843195..4843820 Replicon chromosome
Accession NZ_CP044047
Organism Klebsiella pneumoniae strain FDAARGOS_629

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FOB37_RS25950 Protein ID WP_002882817.1
Coordinates 4843437..4843820 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FOB37_RS25945 Protein ID WP_004150355.1
Coordinates 4843195..4843437 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB37_RS25920 4839186..4839785 + 600 WP_004151865.1 glucose-1-phosphatase -
FOB37_RS25925 4839779..4840639 + 861 WP_004146232.1 virulence factor BrkB family protein -
FOB37_RS25930 4840636..4841073 + 438 WP_064162494.1 D-tyrosyl-tRNA(Tyr) deacylase -
FOB37_RS25935 4841118..4842059 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FOB37_RS25940 4842073..4842990 - 918 WP_004181612.1 alpha/beta hydrolase -
FOB37_RS25945 4843195..4843437 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FOB37_RS25950 4843437..4843820 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FOB37_RS25955 4843994..4844923 - 930 WP_064162495.1 formate dehydrogenase accessory protein FdhE -
FOB37_RS25960 4844920..4845555 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FOB37_RS25965 4845552..4846454 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T139946 WP_002882817.1 NZ_CP044047:4843437-4843820 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T139946 NZ_CP058857:324617-324985 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT139946 WP_004150355.1 NZ_CP044047:4843195-4843437 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT139946 NZ_CP058857:324399-324620 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References