Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 27883..28526 Replicon plasmid unnamed1
Accession NZ_CP044043
Organism Klebsiella pneumoniae strain FDAARGOS_629

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag FOB37_RS00200 Protein ID WP_001034044.1
Coordinates 28110..28526 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag FOB37_RS00195 Protein ID WP_001261286.1
Coordinates 27883..28113 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB37_RS00180 23020..23250 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FOB37_RS00185 23247..23663 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
FOB37_RS00190 23708..27502 - 3795 WP_001144732.1 hypothetical protein -
FOB37_RS00195 27883..28113 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FOB37_RS00200 28110..28526 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
FOB37_RS00205 28601..30166 + 1566 WP_001128474.1 AAA family ATPase -
FOB37_RS00210 30151..31173 + 1023 WP_000361402.1 helicase UvrD -
FOB37_RS00215 31427..32124 - 698 Protein_34 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / dfrA12 / aadA2 / qacE / sul1 / mph(A) / erm(B) iutA / iucD / iucC / iucB / iucA 1..72634 72634


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T139931 WP_001034044.1 NZ_CP044043:28110-28526 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T139931 NZ_CP058853:c2448336-2448233 [Shigella flexneri]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT139931 WP_001261286.1 NZ_CP044043:27883-28113 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT139931 NZ_CP058853:2448195-2448466 [Shigella flexneri]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References