Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 18531..19056 Replicon plasmid unnamed1
Accession NZ_CP044043
Organism Klebsiella pneumoniae strain FDAARGOS_629

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag FOB37_RS00150 Protein ID WP_001159868.1
Coordinates 18531..18836 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FOB37_RS00155 Protein ID WP_000813634.1
Coordinates 18838..19056 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB37_RS00135 14441..15607 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
FOB37_RS00140 16195..16950 - 756 WP_000852146.1 replication initiation protein RepE -
FOB37_RS00145 17724..18530 - 807 WP_000016982.1 site-specific integrase -
FOB37_RS00150 18531..18836 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
FOB37_RS00155 18838..19056 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FOB37_RS00160 19623..20135 + 513 WP_000151784.1 hypothetical protein -
FOB37_RS00165 20169..21302 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
FOB37_RS00170 21469..22242 - 774 WP_000905949.1 hypothetical protein -
FOB37_RS00175 22255..22755 - 501 WP_000528931.1 hypothetical protein -
FOB37_RS00180 23020..23250 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FOB37_RS00185 23247..23663 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / dfrA12 / aadA2 / qacE / sul1 / mph(A) / erm(B) iutA / iucD / iucC / iucB / iucA 1..72634 72634


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T139929 WP_001159868.1 NZ_CP044043:c18836-18531 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T139929 NZ_CP058853:2018645-2018821 [Shigella flexneri]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT139929 WP_000813634.1 NZ_CP044043:c19056-18838 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT139929 NZ_CP058853:2018867-2019283 [Shigella flexneri]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTAGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References