Detailed information of TA system TA139909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 60588..61113
• Replicon: plasmid unnamed4
• Accession: NZ_CP044038
• Organism: Klebsiella pneumoniae strain FDAARGOS_630

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: FOB38_RS02180
• Protein ID: WP_101867324.1
• Coordinates: 60808..61113 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: FOB38_RS02175
• Protein ID: WP_001568025.1
• Coordinates: 60588..60806 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB38_RS02125	56651..56974	+	324	WP_118860658.1	hypothetical protein	-
FOB38_RS02130	57377..57631	-	255	WP_150388104.1	hypothetical protein	-
FOB38_RS02135	57748..57978	-	231	WP_150388105.1	hypothetical protein	-
FOB38_RS02140	57992..58195	-	204	WP_150388106.1	hemolysin expression modulator Hha	-
FOB38_RS02145	58229..58597	-	369	WP_114499706.1	hypothetical protein	-
FOB38_RS02150	58641..59135	-	495	WP_150388107.1	DNA-binding protein	-
FOB38_RS02155	59166..59732	-	567	WP_029497543.1	hypothetical protein	-
FOB38_RS02160	59729..59992	-	264	WP_043519726.1	hypothetical protein	-
FOB38_RS02165	60167..60418	+	252	Protein_76	hypothetical protein	-
FOB38_RS02175	60588..60806	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
FOB38_RS02180	60808..61113	+	306	WP_101867324.1	type II toxin-antitoxin system toxin CcdB	Toxin
FOB38_RS02185	61282..61677	+	396	WP_190932579.1	hypothetical protein	-
FOB38_RS02190	61704..62027	+	324	WP_004197641.1	hypothetical protein	-
FOB38_RS02195	62024..63040	+	1017	WP_017899884.1	hypothetical protein	-
FOB38_RS02200	63238..64032	+	795	WP_150388108.1	site-specific integrase	-
FOB38_RS02205	64464..64643	-	180	WP_006788217.1	hypothetical protein	-
FOB38_RS02210	64763..65389	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..77529	77529

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11638.33 Da
• Isoelectric Point: 6.4674

>T139909 WP_101867324.1 NZ_CP044038:60808-61113 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T139909 NZ_CP058849:2297479-2298801 [Shigella flexneri]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT139909 WP_001568025.1 NZ_CP044038:60588-60806 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT139909 NZ_CP058849:2297213-2297479 [Shigella flexneri]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZZP8