Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 53208..53959 Replicon plasmid unnamed2
Accession NZ_CP044036
Organism Klebsiella pneumoniae strain FDAARGOS_630

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag FOB38_RS01050 Protein ID WP_014386536.1
Coordinates 53477..53959 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag FOB38_RS01045 Protein ID WP_004902250.1
Coordinates 53208..53486 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB38_RS01010 48851..49276 - 426 WP_000522996.1 organomercurial transporter MerC -
FOB38_RS01015 49304..49579 - 276 WP_000732275.1 mercury resistance system periplasmic binding protein MerP -
FOB38_RS01020 49595..49960 - 366 WP_001294656.1 mercuric ion transporter MerT -
FOB38_RS01025 50032..50487 + 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
FOB38_RS01030 51140..51598 + 459 WP_014386535.1 hypothetical protein -
FOB38_RS01035 52428..52769 + 342 WP_004902257.1 hypothetical protein -
FOB38_RS01040 52877..53089 + 213 WP_004902255.1 hypothetical protein -
FOB38_RS01045 53208..53486 + 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
FOB38_RS01050 53477..53959 + 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
FOB38_RS01065 54994..55533 + 540 WP_004902239.1 hypothetical protein -
FOB38_RS01070 55638..56030 + 393 WP_032442757.1 hypothetical protein -
FOB38_RS01075 56131..56760 + 630 Protein_66 DUF2971 domain-containing protein -
FOB38_RS01080 56785..57753 + 969 WP_000654811.1 IS5 family transposase -
FOB38_RS01090 57978..58625 + 648 WP_014386537.1 EcsC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA1 / ARR-3 / dfrA5 / ere(A) / qacE / sul1 / tet(A) / ere(B) - 1..151028 151028


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T139908 WP_014386536.1 NZ_CP044036:53477-53959 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T139908 NZ_CP058849:2243179-2243466 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT139908 WP_004902250.1 NZ_CP044036:53208-53486 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT139908 NZ_CP058849:2242940-2243179 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References