Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 8746..9549 Replicon plasmid unnamed1
Accession NZ_CP044035
Organism Klebsiella pneumoniae strain FDAARGOS_630

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag FOB38_RS00065 Protein ID WP_016479963.1
Coordinates 9019..9549 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag FOB38_RS00060 Protein ID WP_022652312.1
Coordinates 8746..9015 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB38_RS00045 5232..5384 - 153 Protein_7 IS5/IS1182 family transposase -
FOB38_RS00050 5866..6605 + 740 Protein_8 site-specific integrase -
FOB38_RS00055 6901..7890 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
FOB38_RS00060 8746..9015 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
FOB38_RS00065 9019..9549 + 531 WP_016479963.1 GNAT family N-acetyltransferase Toxin
FOB38_RS00070 9681..10697 + 1017 WP_087728544.1 IS5-like element IS5 family transposase -
FOB38_RS00075 10844..11593 - 750 Protein_13 IS3 family transposase -
FOB38_RS00080 11603..11722 - 120 Protein_14 IS30 family transposase -
FOB38_RS00085 11981..12157 - 177 Protein_15 IS91 family transposase -
FOB38_RS00090 12346..13986 - 1641 WP_004201176.1 chaperonin GroEL -
FOB38_RS00095 14042..14332 - 291 WP_004201172.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / rmtC / sul1 htpB 1..114306 114306
- inside IScluster/Tn - - 3265..11509 8244


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T139907 WP_016479963.1 NZ_CP044035:9019-9549 [Klebsiella pneumoniae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T139907 NZ_CP058849:1961586-1961689 [Shigella flexneri]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT139907 WP_022652312.1 NZ_CP044035:8746-9015 [Klebsiella pneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT139907 NZ_CP058849:c1961727-1961456 [Shigella flexneri]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0L7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References