Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1262555..1263180 Replicon chromosome
Accession NZ_CP044033
Organism Klebsiella pneumoniae strain FDAARGOS_631

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FOB39_RS06395 Protein ID WP_002882817.1
Coordinates 1262555..1262938 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FOB39_RS06400 Protein ID WP_004150355.1
Coordinates 1262938..1263180 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB39_RS06380 1259921..1260823 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FOB39_RS06385 1260820..1261455 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FOB39_RS06390 1261452..1262381 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FOB39_RS06395 1262555..1262938 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FOB39_RS06400 1262938..1263180 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FOB39_RS06405 1263385..1264302 + 918 WP_115699160.1 alpha/beta hydrolase -
FOB39_RS06410 1264317..1265258 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FOB39_RS06415 1265303..1265740 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FOB39_RS06420 1265737..1266597 - 861 WP_002882807.1 virulence factor BrkB family protein -
FOB39_RS06425 1266591..1267190 - 600 WP_004146231.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T139892 WP_002882817.1 NZ_CP044033:c1262938-1262555 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T139892 NZ_CP058846:2571184-2571462 [Shigella flexneri]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGCTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT139892 WP_004150355.1 NZ_CP044033:c1263180-1262938 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT139892 NZ_CP058846:2570991-2571182 [Shigella flexneri]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References