Detailed information of TA system TA139889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 671292..672102
• Replicon: chromosome
• Accession: NZ_CP044033
• Organism: Klebsiella pneumoniae strain FDAARGOS_631

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: FOB39_RS03585
• Protein ID: WP_065810545.1
• Coordinates: 671292..671825 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: FOB39_RS03590
• Protein ID: WP_002887278.1
• Coordinates: 671836..672102 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB39_RS03580	670123..671244	+	1122	WP_002887282.1	cupin domain-containing protein	-
FOB39_RS03585	671292..671825	-	534	WP_065810545.1	GNAT family N-acetyltransferase	Toxin
FOB39_RS03590	671836..672102	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
FOB39_RS03595	672205..673638	-	1434	WP_065810546.1	Cu(+)/Ag(+) sensor histidine kinase	-
FOB39_RS03600	673628..674311	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
FOB39_RS03605	674483..675865	+	1383	WP_065810547.1	efflux transporter outer membrane subunit	-
FOB39_RS03610	675883..676227	+	345	WP_025862174.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19770.66 Da
• Isoelectric Point: 5.2612

>T139889 WP_065810545.1 NZ_CP044033:c671825-671292 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPKLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGCIQLMDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T139889 NZ_CP058846:1990770-1990946 [Shigella flexneri]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT139889 WP_002887278.1 NZ_CP044033:c672102-671836 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT139889 NZ_CP058846:1990992-1991408 [Shigella flexneri]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1