Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 128397..129040 Replicon plasmid pY9645-166
Accession NZ_CP044029
Organism Klebsiella pneumoniae strain RJY9645

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag FJR44_RS26020 Protein ID WP_008322233.1
Coordinates 128397..128813 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag FJR44_RS26025 Protein ID WP_001261276.1
Coordinates 128810..129040 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJR44_RS26000 124467..125486 + 1020 WP_004113181.1 ribose ABC transporter permease -
FJR44_RS26005 125564..126934 + 1371 WP_008322237.1 hypothetical protein -
FJR44_RS26010 126931..127851 + 921 WP_008322235.1 carbohydrate kinase -
FJR44_RS26015 128034..128203 - 170 Protein_140 DUF596 domain-containing protein -
FJR44_RS26020 128397..128813 - 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
FJR44_RS26025 128810..129040 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FJR44_RS26030 129293..130315 - 1023 WP_008322227.1 helicase UvrD -
FJR44_RS26035 130401..131105 + 705 WP_001067848.1 IS6-like element IS26 family transposase -
FJR44_RS26040 131508..131864 + 357 WP_000215515.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / qnrS1 / mph(A) / sul1 / qacE / aadA2 / dfrA12 - 1..166344 166344


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T139887 WP_008322233.1 NZ_CP044029:c128813-128397 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T139887 NZ_CP058846:c1772853-1772746 [Shigella flexneri]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT139887 WP_001261276.1 NZ_CP044029:c129040-128810 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT139887 NZ_CP058846:1772900-1772966 [Shigella flexneri]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References