Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 13189..13992 Replicon plasmid pY9645-166
Accession NZ_CP044029
Organism Klebsiella pneumoniae strain RJY9645

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag FJR44_RS25385 Protein ID WP_022652313.1
Coordinates 13462..13992 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag FJR44_RS25380 Protein ID WP_022652312.1
Coordinates 13189..13458 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJR44_RS25365 9494..9801 - 308 Protein_10 IS1 family transposase -
FJR44_RS25370 10278..11018 + 741 WP_022652310.1 site-specific integrase -
FJR44_RS25375 11344..12333 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
FJR44_RS25380 13189..13458 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
FJR44_RS25385 13462..13992 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
FJR44_RS25390 14124..15017 + 894 WP_077873527.1 IS5 family transposase -
FJR44_RS25395 15158..16054 - 897 Protein_16 IS3 family transposase -
FJR44_RS25400 16119..17270 + 1152 WP_022652315.1 IS30-like element IS30 family transposase -
FJR44_RS25405 17379..17762 + 384 WP_001201739.1 IS66 family insertion sequence hypothetical protein -
FJR44_RS25410 17759..18106 + 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / qnrS1 / mph(A) / sul1 / qacE / aadA2 / dfrA12 - 1..166344 166344
- inside IScluster/Tn - - 8180..21561 13381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T139886 WP_022652313.1 NZ_CP044029:13462-13992 [Klebsiella pneumoniae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T139886 NZ_CP058846:c1772318-1772211 [Shigella flexneri]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT139886 WP_022652312.1 NZ_CP044029:13189-13458 [Klebsiella pneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT139886 NZ_CP058846:1772365-1772431 [Shigella flexneri]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References