Detailed information of TA system TA139878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 221502..222082
• Replicon: plasmid pEMPC1
• Accession: NZ_CP044012
• Organism: Sinorhizobium sp. BG8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F3Y30_RS22140
• Protein ID: WP_203427330.1
• Coordinates: 221699..222082 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: F3Y30_RS22135
• Protein ID: WP_203427329.1
• Coordinates: 221502..221702 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3Y30_RS22110	217189..217767	+	579	WP_203427326.1	L,D-transpeptidase	-
F3Y30_RS22115	218325..218915	+	591	WP_203427603.1	hypothetical protein	-
F3Y30_RS22120	218978..219571	+	594	WP_203427327.1	isoprenylcysteine carboxylmethyltransferase family protein	-
F3Y30_RS22125	219581..220366	+	786	WP_203427328.1	hypothetical protein	-
F3Y30_RS22130	220363..221228	+	866	Protein_206	SDR family NAD(P)-dependent oxidoreductase	-
F3Y30_RS22135	221502..221702	+	201	WP_203427329.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
F3Y30_RS22140	221699..222082	+	384	WP_203427330.1	type II toxin-antitoxin system VapC family toxin	Toxin
F3Y30_RS22145	222170..222664	-	495	Protein_209	ribose ABC transporter	-
F3Y30_RS22150	222738..223058	-	321	WP_203427331.1	TOBE domain-containing protein	-
F3Y30_RS22155	223036..223455	-	420	WP_203427332.1	hypothetical protein	-
F3Y30_RS22160	223455..223751	+	297	WP_203427333.1	LrgB family protein	-
F3Y30_RS22165	224171..225439	+	1269	Protein_213	SfnB family sulfur acquisition oxidoreductase	-
F3Y30_RS22170	225776..226378	-	603	WP_203427334.1	carbonic anhydrase	-
F3Y30_RS22175	226479..226670	-	192	WP_203427335.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..1138596	1138596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14102.21 Da
• Isoelectric Point: 6.3269

>T139878 WP_203427330.1 NZ_CP044012:221699-222082 [Sinorhizobium sp. BG8]
VILADTSIWIDHFRRGDAELRRIIEDDRLLCHPAVIGELALGSLRDRGSVIAFLAAQREAFVATHDEVMMMIDRHAIFSM
GIGYTDAHLLASILLDQRAALWTRDKRLWAAAEKAGALLYTSANSGN

Nucleotide

• Length: 384 bp

>T139878 NZ_CP058842:10017-10298 [Shigella flexneri]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7109.21 Da
• Isoelectric Point: 10.4525

>AT139878 WP_203427329.1 NZ_CP044012:221502-221702 [Sinorhizobium sp. BG8]
MRSTINLDDTLMEKARSLTGIKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAE

Nucleotide

• Length: 201 bp

>AT139878 NZ_CP058842:9776-10027 [Shigella flexneri]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA