Detailed information of TA system TA139875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 536398..536956
• Replicon: chromosome
• Accession: NZ_CP044009
• Organism: Rhizobium sp. BG6

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: F3Y33_RS02660
• Protein ID: WP_153269565.1
• Coordinates: 536398..536661 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: F3Y33_RS02665
• Protein ID: WP_153269564.1
• Coordinates: 536642..536956 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3Y33_RS02635	531401..531847	+	447	WP_153269570.1	DoxX family protein	-
F3Y33_RS02640	531928..532623	-	696	WP_153269569.1	ATP phosphoribosyltransferase	-
F3Y33_RS02645	532623..533741	-	1119	WP_153269568.1	ATP phosphoribosyltransferase regulatory subunit	-
F3Y33_RS02650	533922..535441	-	1520	Protein_526	histidine--tRNA ligase	-
F3Y33_RS02655	535577..536332	+	756	WP_153269566.1	hypothetical protein	-
F3Y33_RS02660	536398..536661	+	264	WP_153269565.1	BrnT family toxin	Toxin
F3Y33_RS02665	536642..536956	+	315	WP_153269564.1	BrnA antitoxin family protein	Antitoxin
F3Y33_RS02670	537142..537288	-	147	WP_203437282.1	hypothetical protein	-
F3Y33_RS02675	537455..537814	+	360	WP_153269562.1	DUF305 domain-containing protein	-
F3Y33_RS02680	537858..538880	-	1023	WP_153269561.1	aldo/keto reductase	-
F3Y33_RS02685	539120..539822	+	703	Protein_533	HAD family hydrolase	-
F3Y33_RS02690	539823..540314	-	492	WP_153269559.1	hypothetical protein	-
F3Y33_RS02695	540311..540940	-	630	WP_153269558.1	DNA-3-methyladenine glycosylase I	-
F3Y33_RS02700	541152..541898	+	747	WP_153269557.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-101)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9685.21 Da
• Isoelectric Point: 5.8355

>T139875 WP_153269565.1 NZ_CP044009:536398-536661 [Rhizobium sp. BG6]
MKIVWDEPKRLANLDKHGLDFADLDEVFFLGAILQPAKRGRLMAIGRFADGSIAVIFAVLGSQGISIVSMRPARKSERRL
LDDSEED

Nucleotide

• Length: 264 bp

>T139875 NZ_CP058839:4083546-4083944 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12021.51 Da
• Isoelectric Point: 8.5442

>AT139875 WP_153269564.1 NZ_CP044009:536642-536956 [Rhizobium sp. BG6]
MTAKKTELQIEFKEGRGYSKEDWDDVSDSPEWTEEDFKNARPFAEVFPELAESIRRSRGRPAVEQPKRQISLRLDPDVID
AFKATGKGWQGRINEALRKAAKLR

Nucleotide

• Length: 315 bp

>AT139875 NZ_CP058839:4083250-4083543 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA