Detailed information of TA system TA139869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3746379..3747600
• Replicon: chromosome
• Accession: NZ_CP044006
• Organism: Pseudomonas aeruginosa strain E90

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FVA82_RS17750
• Protein ID: WP_003160088.1
• Coordinates: 3747022..3747600 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FVA82_RS17745
• Protein ID: WP_003088112.1
• Coordinates: 3746379..3746933 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA82_RS17720	3741947..3742498	+	552	WP_003102727.1	XRE family transcriptional regulator	-
FVA82_RS17725	3742495..3742893	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
FVA82_RS17730	3742906..3743229	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
FVA82_RS17735	3743560..3744021	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
FVA82_RS17740	3744026..3746221	+	2196	WP_031690533.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
FVA82_RS17745	3746379..3746933	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
FVA82_RS17750	3747022..3747600	+	579	WP_003160088.1	HD domain-containing protein	Toxin
FVA82_RS17755	3747709..3748896	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
FVA82_RS17760	3748886..3751057	-	2172	WP_009876279.1	type I secretion system permease/ATPase	-
FVA82_RS17765	3751047..3752324	-	1278	WP_003122808.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21816.65 Da
• Isoelectric Point: 5.1625

>T139869 WP_003160088.1 NZ_CP044006:3747022-3747600 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQCLAARRDA

Nucleotide

• Length: 579 bp

>T139869 NZ_CP058839:c2355798-2355484 [Shigella flexneri]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT139869 WP_003088112.1 NZ_CP044006:3746379-3746933 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT139869 NZ_CP058839:c2355487-2355071 [Shigella flexneri]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA