Detailed information of TA system TA139863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3771511..3772040
• Replicon: chromosome
• Accession: NZ_CP043997
• Organism: Mycobacterium tuberculosis strain 1-0066P6C4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: FPJ49_RS17580
• Protein ID: WP_003417760.1
• Coordinates: 3771783..3772040 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: FPJ49_RS17575
• Protein ID: WP_003417757.1
• Coordinates: 3771511..3771786 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ49_RS17555	3768309..3769746	-	1438	Protein_3467	FAD-binding oxidoreductase	-
FPJ49_RS17560	3769849..3770238	+	390	WP_003417745.1	DUF732 domain-containing protein	-
FPJ49_RS17565	3770252..3770545	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
FPJ49_RS17570	3770542..3771387	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
FPJ49_RS17575	3771511..3771786	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
FPJ49_RS17580	3771783..3772040	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
FPJ49_RS17585	3772082..3773272	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
FPJ49_RS17590	3773389..3773757	+	369	WP_003417765.1	FHA domain-containing protein	-
FPJ49_RS17595	3773754..3774305	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
FPJ49_RS17600	3774312..3774893	-	582	WP_003417769.1	ATP/GTP-binding protein	-
FPJ49_RS17605	3774874..3775242	-	369	WP_003417772.1	DUF742 domain-containing protein	-
FPJ49_RS17610	3775220..3775612	-	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T139863 WP_003417760.1 NZ_CP043997:3771783-3772040 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T139863 NZ_CP058839:c1169296-1169042 [Shigella flexneri]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT139863 WP_003417757.1 NZ_CP043997:3771511-3771786 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT139863 NZ_CP058839:c1169544-1169293 [Shigella flexneri]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94