Detailed information of TA system TA139860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3170597..3171284
• Replicon: chromosome
• Accession: NZ_CP043997
• Organism: Mycobacterium tuberculosis strain 1-0066P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: FPJ49_RS14995
• Protein ID: WP_003414624.1
• Coordinates: 3170841..3171284 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: FPJ49_RS14990
• Protein ID: WP_003414620.1
• Coordinates: 3170597..3170854 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ49_RS14970	3165917..3166771	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
FPJ49_RS14975	3166827..3167990	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FPJ49_RS14980	3168007..3169221	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
FPJ49_RS14985	3169229..3170470	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
FPJ49_RS14990	3170597..3170854	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ49_RS14995	3170841..3171284	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ49_RS15000	3171364..3172026	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
FPJ49_RS15005	3172122..3172313	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ49_RS15010	3172645..3174393	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
FPJ49_RS15015	3174489..3175070	+	582	WP_003414644.1	fasciclin domain-containing protein	-
FPJ49_RS15020	3175170..3175436	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T139860 WP_003414624.1 NZ_CP043997:3170841-3171284 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T139860 NZ_CP058839:c716626-716339 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT139860 WP_003414620.1 NZ_CP043997:3170597-3170854 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT139860 NZ_CP058839:c716865-716626 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0