Detailed information of TA system TA139857

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 3121168..3122942
• Replicon: chromosome
• Accession: NZ_CP043997
• Organism: Mycobacterium tuberculosis strain 1-0066P6C4

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: P71626
• Locus tag: FPJ49_RS14745
• Protein ID: WP_003414409.1
• Coordinates: 3121168..3122052 (-)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: P71625
• Locus tag: FPJ49_RS14750
• Protein ID: WP_003414414.1
• Coordinates: 3122055..3122942 (-)
• Length: 296 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ49_RS14725	3116423..3116797	-	375	WP_003414296.1	type III-A CRISPR-associated protein Csm2	-
FPJ49_RS14730	3116794..3119232	-	2439	WP_003900580.1	type III-A CRISPR-associated protein Cas10/Csm1	-
FPJ49_RS14735	3119229..3120017	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
FPJ49_RS14740	3120351..3120896	-	546	WP_014584866.1	DUF1802 family protein	-
FPJ49_RS14745	3121168..3122052	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
FPJ49_RS14750	3122055..3122942	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
FPJ49_RS14755	3123247..3123792	-	546	WP_003910939.1	DUF1802 family protein	-
FPJ49_RS14760	3123789..3124058	-	270	WP_003414489.1	DUF2277 family protein	-
FPJ49_RS14765	3124079..3124471	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	-
FPJ49_RS14770	3124468..3124683	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ49_RS14775	3124730..3125479	+	750	WP_003902358.1	enoyl-CoA hydratase	-
FPJ49_RS14780	3125558..3126640	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-272)

Antitoxin

• AbiEi antitoxin C-terminal domain (84-237)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 33056.72 Da
• Isoelectric Point: 7.1520

>T139857 WP_003414409.1 NZ_CP043997:c3122052-3121168 [Mycobacterium tuberculosis]
VAGLTRALVARHALGRAEAYDAALLDVAQDHLLYLLSQTVQFGDNRLVFKGGTSLRKCRLGNVGRFSTDLDFSAPDDEVV
LEVCELIDGARVGGFEFGVQSTRGDGRHWQLRVRHTELGEPRIVASVEFARRPLALPSELLAFIQLPIHKAYGFGLPTLP
VVAEAEACAEKLARYRRVALARDLYDLNHFASRTIDEPLVRRLWVLKVWGDVVDDRRGTRPLRVEDVLAARSEHDFQPDS
IGVLTRPVAMAAWEARVRKRFAFLTDLDADEQRWAACDERHRREVENALAVLRS

Nucleotide

• Length: 885 bp

>T139857 NZ_CP058839:c365683-365576 [Shigella flexneri]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 296 a.a.
• Molecular weight: 32315.19 Da
• Isoelectric Point: 9.4723

>AT139857 WP_003414414.1 NZ_CP043997:c3122942-3122055 [Mycobacterium tuberculosis]
VVSPAGADRRIPTWASRVVSGLARDRPVVVTKEDLTQRLTEAGCGRDPDSAIRELRRIGWLVQLPVKGTWAFIPPGEAAI
SDPYLPLRSWLARDQNAGFMLAGASAAWHLGYLDRQPDGRIPIWLPPAKRLPDGLASYVSVVRIPWNAADTALLAPRPAL
LVRRRLDLVAWATGLPALGPEALLVQIATRPASFGPWADLVPHLDDLVADCSDERLERLLSGRPTSAWQRASYLLDSGGE
PARGQALLAKRHTEVMPVTRFTTAHSRDRGESVWAPEYQLVDELVVPLLRVIGKA

Nucleotide

• Length: 888 bp

>AT139857 NZ_CP058839:365730-365796 [Shigella flexneri]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• PDB: 6Y56

Antitoxin

• AlphaFold DB: A0A829CBU9