Detailed information of TA system TA139852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2924892..2925606
• Replicon: chromosome
• Accession: NZ_CP043997
• Organism: Mycobacterium tuberculosis strain 1-0066P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: FPJ49_RS13600
• Protein ID: WP_003413460.1
• Coordinates: 2925166..2925606 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: FPJ49_RS13595
• Protein ID: WP_003413456.1
• Coordinates: 2924892..2925179 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ49_RS13560	2920314..2920559	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FPJ49_RS13565	2920556..2920960	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
FPJ49_RS13570	2921177..2921797	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
FPJ49_RS13575	2921808..2922302	+	495	WP_003413444.1	DUF2617 family protein	-
FPJ49_RS13580	2922299..2922730	+	432	WP_003899390.1	DUF4247 domain-containing protein	-
FPJ49_RS13585	2922755..2923213	+	459	WP_003413451.1	DUF350 domain-containing protein	-
FPJ49_RS13590	2923210..2924781	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
FPJ49_RS13595	2924892..2925179	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
FPJ49_RS13600	2925166..2925606	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ49_RS13605	2925627..2926382	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
FPJ49_RS13610	2926515..2927111	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
FPJ49_RS13615	2927119..2927964	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
FPJ49_RS13620	2927993..2928892	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
FPJ49_RS13625	2929020..2929694	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T139852 WP_003413460.1 NZ_CP043997:2925166-2925606 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T139852 NZ_CP058833:4084752-4085150 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT139852 WP_003413456.1 NZ_CP043997:2924892-2925179 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT139852 NZ_CP058833:4084456-4084749 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8