Detailed information of TA system TA139850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2863429..2864060
• Replicon: chromosome
• Accession: NZ_CP043997
• Organism: Mycobacterium tuberculosis strain 1-0066P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPJ49_RS13325
• Protein ID: WP_003413174.1
• Coordinates: 2863683..2864060 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ49_RS13320
• Protein ID: WP_003413167.1
• Coordinates: 2863429..2863686 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ49_RS13290	2859344..2859658	+	315	WP_009937839.1	hypothetical protein	-
FPJ49_RS13295	2859954..2861165	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ49_RS13300	2861292..2861951	+	660	WP_003902296.1	hypothetical protein	-
FPJ49_RS13305	2861948..2862610	+	663	WP_003905878.1	hypothetical protein	-
FPJ49_RS13310	2862607..2862884	+	278	Protein_2627	type II toxin-antitoxin system VapB family antitoxin	-
FPJ49_RS13315	2862977..2863390	+	414	WP_003413164.1	PIN domain nuclease	-
FPJ49_RS13320	2863429..2863686	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ49_RS13325	2863683..2864060	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ49_RS13330	2864076..2864450	-	375	WP_003413177.1	hypothetical protein	-
FPJ49_RS13335	2864550..2864945	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ49_RS13340	2864942..2865187	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ49_RS13345	2865598..2866017	-	420	WP_003413190.1	A24 family peptidase	-
FPJ49_RS13350	2866029..2866838	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ49_RS13355	2866835..2868088	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ49_RS13360	2868081..2868593	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T139850 WP_003413174.1 NZ_CP043997:2863683-2864060 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T139850 NZ_CP058833:3723731-3724018 [Shigella flexneri]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT139850 WP_003413167.1 NZ_CP043997:2863429-2863686 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT139850 NZ_CP058833:3724005-3724307 [Shigella flexneri]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5