Detailed information of TA system TA139840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2225104..2226002
• Replicon: chromosome
• Accession: NZ_CP043997
• Organism: Mycobacterium tuberculosis strain 1-0066P6C4

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: FPJ49_RS10340
• Protein ID: WP_003410001.1
• Coordinates: 2225104..2225664 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: FPJ49_RS10345
• Protein ID: WP_003410003.1
• Coordinates: 2225661..2226002 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ49_RS10310	2220273..2220926	-	654	WP_003409976.1	cutinase Cfp21	-
FPJ49_RS10315	2221041..2221271	+	231	WP_003409980.1	DUF167 domain-containing protein	-
FPJ49_RS10320	2221356..2222267	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
FPJ49_RS10325	2222376..2222975	+	600	WP_003910883.1	L-lysine exporter	-
FPJ49_RS10330	2223391..2223819	+	429	WP_003409992.1	cellulose-binding protein	-
FPJ49_RS10335	2224045..2224584	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
FPJ49_RS10340	2225104..2225664	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
FPJ49_RS10345	2225661..2226002	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
FPJ49_RS10350	2226088..2226345	+	258	WP_003410006.1	hypothetical protein	-
FPJ49_RS10355	2226246..2226581	-	336	WP_003410009.1	dehydrogenase	-
FPJ49_RS10360	2226670..2227014	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
FPJ49_RS10365	2227008..2227256	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
FPJ49_RS10370	2227356..2229671	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
FPJ49_RS10375	2229668..2229940	-	273	WP_003410017.1	DUF1490 family protein	-
FPJ49_RS10380	2229993..2230349	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T139840 WP_003410001.1 NZ_CP043997:c2225664-2225104 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T139840 NZ_CP058833:c1169296-1169042 [Shigella flexneri]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT139840 WP_003410003.1 NZ_CP043997:c2226002-2225661 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT139840 NZ_CP058833:c1169544-1169293 [Shigella flexneri]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 6FKG

Antitoxin

• AlphaFold DB: A0A7U4FAE4