Detailed information of TA system TA139827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240366..1240934
• Replicon: chromosome
• Accession: NZ_CP043997
• Organism: Mycobacterium tuberculosis strain 1-0066P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FPJ49_RS05865
• Protein ID: WP_003405865.1
• Coordinates: 1240560..1240934 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPJ49_RS05860
• Protein ID: WP_003405863.1
• Coordinates: 1240366..1240563 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ49_RS05840	1236407..1237045	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPJ49_RS05845	1237135..1238142	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPJ49_RS05850	1238159..1239142	-	984	WP_003898731.1	hypothetical protein	-
FPJ49_RS05855	1239205..1240278	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FPJ49_RS05860	1240366..1240563	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ49_RS05865	1240560..1240934	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ49_RS05870	1241137..1241835	+	699	WP_003898733.1	hypothetical protein	-
FPJ49_RS05875	1241953..1242138	+	186	WP_003901093.1	hypothetical protein	-
FPJ49_RS05880	1242065..1242340	-	276	WP_003405867.1	hypothetical protein	-
FPJ49_RS05885	1242583..1242906	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPJ49_RS05890	1242921..1243631	-	711	WP_003911399.1	hypothetical protein	-
FPJ49_RS05895	1243653..1243862	+	210	WP_003911400.1	hypothetical protein	-
FPJ49_RS05900	1243814..1244521	-	708	Protein_1162	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T139827 WP_003405865.1 NZ_CP043997:1240560-1240934 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T139827 NZ_CP058831:3637382-3637660 [Shigella flexneri]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT139827 WP_003405863.1 NZ_CP043997:1240366-1240563 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT139827 NZ_CP058831:3637119-3637379 [Shigella flexneri]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07