Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3180753..3181384 Replicon chromosome
Accession NZ_CP043995
Organism Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag FPJ87_RS14895 Protein ID WP_003898728.1
Coordinates 3181073..3181384 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag FPJ87_RS14890 Protein ID WP_003405836.1
Coordinates 3180753..3181073 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ87_RS14865 3175949..3176587 + 639 WP_003898730.1 lipid droplet-associated protein -
FPJ87_RS14870 3176584..3177831 + 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
FPJ87_RS14875 3177821..3178078 + 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
FPJ87_RS14880 3178088..3179200 + 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
FPJ87_RS14885 3179218..3180743 - 1526 Protein_2938 carboxylesterase/lipase family protein -
FPJ87_RS14890 3180753..3181073 + 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
FPJ87_RS14895 3181073..3181384 + 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
FPJ87_RS14900 3181496..3182653 + 1158 WP_003898727.1 AI-2E family transporter -
FPJ87_RS14905 3182660..3183304 - 645 WP_003915494.1 DUF4245 domain-containing protein -
FPJ87_RS14910 3183360..3184448 + 1089 WP_003898726.1 class II fructose-bisphosphatase -
FPJ87_RS14915 3184479..3185903 + 1425 WP_003405805.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T139737 WP_003898728.1 NZ_CP043995:3181073-3181384 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T139737 NZ_CP058790:3744446-3744724 [Shigella flexneri]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT139737 WP_003405836.1 NZ_CP043995:3180753-3181073 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT139737 NZ_CP058790:3744183-3744443 [Shigella flexneri]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References