Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2726450..2727066 Replicon chromosome
Accession NZ_CP043995
Organism Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag FPJ87_RS12855 Protein ID WP_003407593.1
Coordinates 2726450..2726767 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag FPJ87_RS12860 Protein ID WP_003900349.1
Coordinates 2726764..2727066 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ87_RS12825 2721459..2722487 - 1029 WP_003407612.1 glycosyltransferase family 2 protein -
FPJ87_RS12830 2722757..2722981 + 225 WP_160522526.1 NUDIX domain-containing protein -
FPJ87_RS12835 2722991..2723203 + 213 WP_003898900.1 dodecin family protein -
FPJ87_RS12840 2723261..2724034 + 774 WP_003912632.1 class I SAM-dependent methyltransferase -
FPJ87_RS12845 2724107..2725396 - 1290 WP_003407599.1 serine hydrolase -
FPJ87_RS12850 2725449..2726453 - 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
FPJ87_RS12855 2726450..2726767 - 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
FPJ87_RS12860 2726764..2727066 - 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
FPJ87_RS12865 2727080..2729332 - 2253 WP_003407587.1 methylmalonyl-CoA mutase -
FPJ87_RS12870 2729333..2731180 - 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T139731 WP_003407593.1 NZ_CP043995:c2726767-2726450 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T139731 NZ_CP058790:c2458711-2458535 [Shigella flexneri]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT139731 WP_003900349.1 NZ_CP043995:c2727066-2726764 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT139731 NZ_CP058790:c2458489-2458073 [Shigella flexneri]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References