Detailed information of TA system TA139728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2454354..2454814
• Replicon: chromosome
• Accession: NZ_CP043995
• Organism: Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TKL9
• Locus tag: FPJ87_RS11650
• Protein ID: WP_003898996.1
• Coordinates: 2454354..2454602 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FPJ87_RS11655
• Protein ID: WP_009938412.1
• Coordinates: 2454602..2454814 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ87_RS11630	2450528..2451139	+	612	WP_003898999.1	isopentenyl-diphosphate Delta-isomerase	-
FPJ87_RS11635	2451129..2451530	+	402	WP_003408538.1	hypothetical protein	-
FPJ87_RS11640	2451815..2453515	-	1701	WP_003898998.1	serine/threonine protein kinase PknE	-
FPJ87_RS11645	2453609..2454346	-	738	WP_003898997.1	hypothetical protein	-
FPJ87_RS11650	2454354..2454602	-	249	WP_003898996.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ87_RS11655	2454602..2454814	-	213	WP_009938412.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ87_RS11660	2454882..2456564	+	1683	WP_003898994.1	SulP family inorganic anion transporter	-
FPJ87_RS11665	2456578..2456862	-	285	WP_003408522.1	DUF1876 domain-containing protein	-
FPJ87_RS11670	2457149..2458336	+	1188	WP_003898992.1	nitrate transporter NarK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin VapB-like (2-69)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8884.14 Da
• Isoelectric Point: 4.1074

>T139728 WP_003898996.1 NZ_CP043995:c2454602-2454354 [Mycobacterium tuberculosis]
MVIDTSALVAMLNDEPEAQRFEIAVAADHVWLMSTASYPEMATVIETRFGEPGGREPKVSGQPLLYKGDDFACIDIRAVL
AG

Nucleotide

• Length: 249 bp

>T139728 NZ_CP058790:1828905-1829159 [Shigella flexneri]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7665.84 Da
• Isoelectric Point: 7.9917

>AT139728 WP_009938412.1 NZ_CP043995:c2454814-2454602 [Mycobacterium tuberculosis]
MELAARMGETLTQAVVVAVREQLARRTGRTRSISLREELAAIGRRCEALPVLDTRAADTILGYDERGLPA

Nucleotide

• Length: 213 bp

>AT139728 NZ_CP058790:1828657-1828908 [Shigella flexneri]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TKL9