Detailed information of TA system TA139727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2227602..2228305
• Replicon: chromosome
• Accession: NZ_CP043995
• Organism: Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FPJ87_RS10640
• Protein ID: WP_003409778.1
• Coordinates: 2227976..2228305 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FPJ87_RS10635
• Protein ID: WP_003409780.1
• Coordinates: 2227602..2227979 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ87_RS10595	2222760..2222951	+	192	WP_003409876.1	hypothetical protein	-
FPJ87_RS10600	2222972..2223874	+	903	WP_003899097.1	hypothetical protein	-
FPJ87_RS10605	2223885..2224235	+	351	WP_003899096.1	hypothetical protein	-
FPJ87_RS10610	2224349..2224531	+	183	WP_003409870.1	hypothetical protein	-
FPJ87_RS10615	2224524..2224925	-	402	WP_003409869.1	hypothetical protein	-
FPJ87_RS10620	2224989..2225441	+	453	WP_003899095.1	lipoprotein	-
FPJ87_RS10625	2225596..2226960	-	1365	WP_003903691.1	HNH endonuclease	-
FPJ87_RS10630	2227015..2227605	+	591	WP_003409784.1	SEC-C domain-containing protein	-
FPJ87_RS10635	2227602..2227979	+	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FPJ87_RS10640	2227976..2228305	+	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FPJ87_RS10645	2228515..2229285	-	771	WP_003409775.1	SDR family oxidoreductase	-
FPJ87_RS10650	2229282..2230343	-	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FPJ87_RS10655	2230340..2230855	-	516	WP_003409718.1	flavin reductase family protein	-
FPJ87_RS10660	2230852..2231922	-	1071	WP_003899091.1	epoxide hydrolase EphB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T139727 WP_003409778.1 NZ_CP043995:2227976-2228305 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T139727 NZ_CP058790:1828657-1828908 [Shigella flexneri]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT139727 WP_003409780.1 NZ_CP043995:2227602-2227979 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT139727 NZ_CP058790:1828905-1829159 [Shigella flexneri]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8