Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2095120..2095756 Replicon chromosome
Accession NZ_CP043995
Organism Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag FPJ87_RS09970 Protein ID WP_003410654.1
Coordinates 2095120..2095530 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag FPJ87_RS09975 Protein ID WP_003410651.1
Coordinates 2095523..2095756 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ87_RS09950 2090717..2091940 + 1224 WP_003901321.1 class I SAM-dependent methyltransferase -
FPJ87_RS09955 2091886..2093412 - 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
FPJ87_RS09960 2093409..2094035 - 627 WP_003410658.1 precorrin-8X methylmutase -
FPJ87_RS09965 2094045..2095136 - 1092 WP_003900454.1 precorrin-3B synthase -
FPJ87_RS09970 2095120..2095530 - 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
FPJ87_RS09975 2095523..2095756 - 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
FPJ87_RS09980 2095834..2099418 + 3585 WP_003410648.1 cobaltochelatase subunit CobN -
FPJ87_RS09985 2099502..2099906 + 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T139719 WP_003410654.1 NZ_CP043995:c2095530-2095120 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T139719 NZ_CP058787:49329-49631 [Shigella flexneri]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT139719 WP_003410651.1 NZ_CP043995:c2095756-2095523 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT139719 NZ_CP058787:49702-50040 [Shigella flexneri]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References