Detailed information of TA system TA139714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1544843..1545552
• Replicon: chromosome
• Accession: NZ_CP043995
• Organism: Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ26
• Locus tag: FPJ87_RS07390
• Protein ID: WP_003413164.1
• Coordinates: 1545139..1545552 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPJ87_RS07385
• Protein ID: WP_003413167.1
• Coordinates: 1544843..1545100 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ87_RS07345	1539936..1540448	+	513	WP_003413197.1	Holliday junction resolvase RuvX	-
FPJ87_RS07350	1540441..1541694	+	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FPJ87_RS07355	1541691..1542500	+	810	WP_003413193.1	shikimate dehydrogenase	-
FPJ87_RS07360	1542512..1542931	+	420	WP_003413190.1	A24 family peptidase	-
FPJ87_RS07365	1543342..1543587	+	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPJ87_RS07370	1543584..1543979	+	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPJ87_RS07375	1544079..1544453	+	375	WP_003413177.1	hypothetical protein	-
FPJ87_RS07380	1544469..1544846	-	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
FPJ87_RS07385	1544843..1545100	-	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPJ87_RS07390	1545139..1545552	-	414	WP_003413164.1	PIN domain nuclease	Toxin
FPJ87_RS07395	1545645..1545923	-	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ87_RS07400	1545920..1546581	-	662	Protein_1458	hypothetical protein	-
FPJ87_RS07405	1546578..1547237	-	660	WP_003900846.1	LppA family lipoprotein	-
FPJ87_RS07410	1547364..1548575	-	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPJ87_RS07415	1548871..1549185	-	315	WP_009937839.1	hypothetical protein	-
FPJ87_RS07420	1549293..1550498	+	1206	WP_003413027.1	chorismate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15045.00 Da
• Isoelectric Point: 5.4673

>T139714 WP_003413164.1 NZ_CP043995:c1545552-1545139 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEWIVPRGTL

Nucleotide

• Length: 414 bp

>T139714 NZ_CP058786:3491255-3491473 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT139714 WP_003413167.1 NZ_CP043995:c1545100-1544843 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT139714 NZ_CP058786:3490855-3491229 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: G0TQ26

Antitoxin

• AlphaFold DB: A0A829C9W5