Detailed information of TA system TA139709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1344059..1344738
• Replicon: chromosome
• Accession: NZ_CP043995
• Organism: Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FPJ87_RS06285
• Protein ID: WP_003414059.1
• Coordinates: 1344322..1344738 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FPJ87_RS06280
• Protein ID: WP_003414061.1
• Coordinates: 1344059..1344325 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ87_RS06245	1339535..1340272	-	738	WP_003414079.1	dienelactone hydrolase family protein	-
FPJ87_RS06250	1340428..1341228	+	801	WP_009939104.1	thymidylate synthase	-
FPJ87_RS06255	1341299..1341778	+	480	WP_003414073.1	dihydrofolate reductase	-
FPJ87_RS06260	1341852..1342271	+	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FPJ87_RS06265	1342268..1343362	+	1095	WP_003414068.1	restriction endonuclease subunit S	-
FPJ87_RS06270	1343372..1343641	+	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ87_RS06275	1343638..1344033	+	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FPJ87_RS06280	1344059..1344325	+	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPJ87_RS06285	1344322..1344738	+	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FPJ87_RS06290	1344825..1346447	+	1623	WP_003906897.1	type I restriction-modification system subunit M	-
FPJ87_RS06295	1346444..1346719	+	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FPJ87_RS06300	1346963..1347715	+	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FPJ87_RS06305	1347784..1348686	+	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T139709 WP_003414059.1 NZ_CP043995:1344322-1344738 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T139709 NZ_CP058786:2297398-2297685 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT139709 WP_003414061.1 NZ_CP043995:1344059-1344325 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT139709 NZ_CP058786:2297159-2297398 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64