Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1344059..1344738 Replicon chromosome
Accession NZ_CP043995
Organism Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag FPJ87_RS06285 Protein ID WP_003414059.1
Coordinates 1344322..1344738 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FPJ87_RS06280 Protein ID WP_003414061.1
Coordinates 1344059..1344325 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ87_RS06245 1339535..1340272 - 738 WP_003414079.1 dienelactone hydrolase family protein -
FPJ87_RS06250 1340428..1341228 + 801 WP_009939104.1 thymidylate synthase -
FPJ87_RS06255 1341299..1341778 + 480 WP_003414073.1 dihydrofolate reductase -
FPJ87_RS06260 1341852..1342271 + 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FPJ87_RS06265 1342268..1343362 + 1095 WP_003414068.1 restriction endonuclease subunit S -
FPJ87_RS06270 1343372..1343641 + 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FPJ87_RS06275 1343638..1344033 + 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
FPJ87_RS06280 1344059..1344325 + 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FPJ87_RS06285 1344322..1344738 + 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
FPJ87_RS06290 1344825..1346447 + 1623 WP_003906897.1 type I restriction-modification system subunit M -
FPJ87_RS06295 1346444..1346719 + 276 WP_003414055.1 type I restriction endonuclease subunit S -
FPJ87_RS06300 1346963..1347715 + 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FPJ87_RS06305 1347784..1348686 + 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T139709 WP_003414059.1 NZ_CP043995:1344322-1344738 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T139709 NZ_CP058786:2297398-2297685 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT139709 WP_003414061.1 NZ_CP043995:1344059-1344325 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT139709 NZ_CP058786:2297159-2297398 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References