Detailed information of TA system TA139700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 755721..756513
• Replicon: chromosome
• Accession: NZ_CP043995
• Organism: Mycobacterium tuberculosis strain 4-0041P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: FPJ87_RS03470
• Protein ID: WP_003403386.1
• Coordinates: 756076..756513 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FPJ87_RS03465
• Protein ID: WP_003403381.1
• Coordinates: 755721..755966 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ87_RS03435	750741..752246	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FPJ87_RS03440	752327..753337	+	1011	WP_009938249.1	ABC transporter ATP-binding protein	-
FPJ87_RS03445	753725..754108	-	384	WP_003403365.1	ribonuclease VapC6	-
FPJ87_RS03450	754203..754358	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FPJ87_RS03455	754434..755150	-	717	WP_009938832.1	CPBP family intramembrane metalloprotease	-
FPJ87_RS03460	755426..755734	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPJ87_RS03465	755721..755966	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPJ87_RS03470	756076..756513	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPJ87_RS03475	756510..756764	-	255	WP_003911263.1	antitoxin VapB7	-
FPJ87_RS03480	756878..759241	+	2364	WP_003898547.1	arylsulfatase AtsD	-
FPJ87_RS03485	759304..759630	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ87_RS03490	759542..759880	+	339	WP_003403405.1	PIN domain-containing protein	-
FPJ87_RS03495	759877..760050	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T139700 WP_003403386.1 NZ_CP043995:c756513-756076 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T139700 NZ_CP058786:648252-648566 [Shigella flexneri]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT139700 WP_003403381.1 NZ_CP043995:c755966-755721 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT139700 NZ_CP058786:648563-648979 [Shigella flexneri]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0