Detailed information of TA system TA139684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2340475..2341453
• Replicon: chromosome
• Accession: NZ_CP043966
• Organism: Bacillus cereus strain H2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: F3L01_RS11980
• Protein ID: WP_000624977.1
• Coordinates: 2340475..2341212 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: F3L01_RS11985
• Protein ID: WP_000237819.1
• Coordinates: 2341325..2341453 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3L01_RS11955	2336088..2336495	+	408	WP_000063587.1	VOC family protein	-
F3L01_RS11960	2336519..2336899	+	381	WP_002025805.1	hypothetical protein	-
F3L01_RS11965	2337057..2338742	-	1686	WP_002025806.1	alpha-keto acid decarboxylase family protein	-
F3L01_RS11970	2338850..2339332	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
F3L01_RS11975	2339499..2340236	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
F3L01_RS11980	2340475..2341212	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
F3L01_RS11985	2341325..2341453	+	129	WP_000237819.1	hypothetical protein	Antitoxin
F3L01_RS11990	2341526..2341702	+	177	WP_000852629.1	stage II sporulation protein SB	-
F3L01_RS11995	2341722..2342111	-	390	WP_000713862.1	YxeA family protein	-
F3L01_RS12000	2342323..2343792	+	1470	WP_000287555.1	beta-Ala-His dipeptidase	-
F3L01_RS12005	2344038..2344847	+	810	WP_000678368.1	C39 family peptidase	-
F3L01_RS12010	2344873..2345475	+	603	WP_000517274.1	hypothetical protein	-
F3L01_RS12015	2345719..2345970	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T139684 WP_000624977.1 NZ_CP043966:2340475-2341212 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T139684 NZ_CP058780:1764238-1764489 [Shigella flexneri]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT139684 WP_000237819.1 NZ_CP043966:2341325-2341453 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT139684 NZ_CP058780:1764486-1764740 [Shigella flexneri]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2