Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 239913..240555 Replicon chromosome
Accession NZ_CP043966
Organism Bacillus cereus strain H2

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag F3L01_RS01355 Protein ID WP_000635963.1
Coordinates 240205..240555 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag F3L01_RS01350 Protein ID WP_000004570.1
Coordinates 239913..240200 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3L01_RS01325 235230..236192 + 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -
F3L01_RS01330 236185..236757 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
F3L01_RS01335 236851..237210 + 360 WP_000583416.1 holo-ACP synthase -
F3L01_RS01340 237367..238317 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
F3L01_RS01345 238435..239604 + 1170 WP_061685369.1 alanine racemase -
F3L01_RS01350 239913..240200 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
F3L01_RS01355 240205..240555 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
F3L01_RS01360 240623..242791 + 2169 WP_000426245.1 RNA-binding transcriptional accessory protein -
F3L01_RS01365 242849..242965 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
F3L01_RS01370 243161..243619 + 459 WP_000344233.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T139683 WP_000635963.1 NZ_CP043966:240205-240555 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T139683 NZ_CP058780:989387-989722 [Shigella flexneri]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT139683 WP_000004570.1 NZ_CP043966:239913-240200 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT139683 NZ_CP058780:989139-989387 [Shigella flexneri]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References