Detailed information of TA system TA139682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2176353..2176833
• Replicon: chromosome
• Accession: NZ_CP043962
• Organism: Agrobacterium fabrum strain BG5

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A9CK22
• Locus tag: F3P66_RS10520
• Protein ID: WP_010971059.1
• Coordinates: 2176564..2176833 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A9CK21
• Locus tag: F3P66_RS10515
• Protein ID: WP_010971060.1
• Coordinates: 2176353..2176580 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P66_RS10485	2171366..2171800	+	435	WP_010971066.1	hypothetical protein	-
F3P66_RS10490	2171956..2172402	+	447	WP_006313161.1	DoxX family protein	-
F3P66_RS10495	2172467..2173159	-	693	WP_010971064.1	ATP phosphoribosyltransferase	-
F3P66_RS10500	2173156..2174283	-	1128	WP_010971063.1	ATP phosphoribosyltransferase regulatory subunit	-
F3P66_RS10505	2174300..2174668	-	369	WP_010971062.1	VOC family protein	-
F3P66_RS10510	2174673..2176196	-	1524	WP_010971061.1	histidine--tRNA ligase	-
F3P66_RS10515	2176353..2176580	+	228	WP_010971060.1	ribbon-helix-helix protein, CopG family	Antitoxin
F3P66_RS10520	2176564..2176833	+	270	WP_010971059.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F3P66_RS10525	2176840..2177604	+	765	WP_010971058.1	hypothetical protein	-
F3P66_RS10530	2177630..2178547	-	918	WP_010971057.1	LysR family transcriptional regulator	-
F3P66_RS10535	2178629..2179672	+	1044	WP_035256280.1	YeiH family protein	-
F3P66_RS10540	2179698..2180141	-	444	WP_010972617.1	hypothetical protein	-
F3P66_RS10545	2180138..2180767	-	630	WP_010971055.1	DNA-3-methyladenine glycosylase I	-
F3P66_RS10550	2181006..2181782	+	777	WP_010971054.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10746.46 Da
• Isoelectric Point: 9.9414

>T139682 WP_010971059.1 NZ_CP043962:2176564-2176833 [Agrobacterium fabrum]
VIWTIEYHTLVQKEMRKINPEVRRRIRSFLHERLAALDDPRQIGATLQGSELGNFWRYRVGDYRIICDIQDQKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T139682 NZ_CP058780:867673-868080 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8937.97 Da
• Isoelectric Point: 4.7254

>AT139682 WP_010971060.1 NZ_CP043962:2176353-2176580 [Agrobacterium fabrum]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKIISAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT139682 NZ_CP058780:867426-867692 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A9CK22

Antitoxin

• AlphaFold DB: A9CK21