Detailed information of TA system TA139681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 1922356..1922963
• Replicon: chromosome
• Accession: NZ_CP043962
• Organism: Agrobacterium fabrum strain BG5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q7D0B7
• Locus tag: F3P66_RS09230
• Protein ID: WP_010971265.1
• Coordinates: 1922628..1922963 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A9CJP8
• Locus tag: F3P66_RS09225
• Protein ID: WP_010971266.1
• Coordinates: 1922356..1922631 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P66_RS09195	1917512..1917871	-	360	WP_006311244.1	type II toxin-antitoxin system PemK/MazF family toxin	-
F3P66_RS09200	1917871..1918137	-	267	WP_010971270.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
F3P66_RS09205	1918207..1918407	-	201	WP_006311242.1	heavy-metal-associated domain-containing protein	-
F3P66_RS09210	1918460..1918882	-	423	WP_006311241.1	Cu(I)-responsive transcriptional regulator	-
F3P66_RS09215	1918879..1921464	-	2586	WP_035256421.1	heavy metal translocating P-type ATPase	-
F3P66_RS09220	1921640..1922248	-	609	WP_010971267.1	class I SAM-dependent methyltransferase	-
F3P66_RS09225	1922356..1922631	+	276	WP_010971266.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
F3P66_RS09230	1922628..1922963	+	336	WP_010971265.1	type II toxin-antitoxin system YafQ family toxin	Toxin
F3P66_RS09235	1922991..1923815	-	825	WP_010971264.1	class D beta-lactamase	-
F3P66_RS09240	1923927..1924406	-	480	WP_010971263.1	YcgN family cysteine cluster protein	-
F3P66_RS09245	1924663..1926858	+	2196	WP_035256415.1	penicillin-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (23-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-67)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12991.86 Da
• Isoelectric Point: 10.0059

>T139681 WP_010971265.1 NZ_CP043962:1922628-1922963 [Agrobacterium fabrum]
VTNKKDHGKDAALKRATLPRRSDFTKQFIKDWQRLNNSGRYDMVRLKEIMLLLIANGAPLPTQFRDHELTGDWRDHRECH
VGGDFLLIYTVDEKQNLLIFTRAGTHAELFR

Nucleotide

• Length: 336 bp

>T139681 NZ_CP058780:731346-731642 [Shigella flexneri]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9918.40 Da
• Isoelectric Point: 6.3350

>AT139681 WP_010971266.1 NZ_CP043962:1922356-1922631 [Agrobacterium fabrum]
MTANAYVRARIDQTLKDDATAVLDRLGLTVSDVMRMMLTRIAREKALPIELTQPNAETLAAIEEARAIAAAGRNRFGTSE
ALFEALDAGKR

Nucleotide

• Length: 276 bp

>AT139681 NZ_CP058780:731644-732039 [Shigella flexneri]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: Q7D0B7

Antitoxin

• AlphaFold DB: A9CJP8