Detailed information of TA system TA139680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 1917512..1918137
• Replicon: chromosome
• Accession: NZ_CP043962
• Organism: Agrobacterium fabrum strain BG5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q7D0B1
• Locus tag: F3P66_RS09195
• Protein ID: WP_006311244.1
• Coordinates: 1917512..1917871 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q7D0B2
• Locus tag: F3P66_RS09200
• Protein ID: WP_010971270.1
• Coordinates: 1917871..1918137 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3P66_RS09175	1913650..1915368	+	1719	WP_035256424.1	glycoside hydrolase family 32 protein	-
F3P66_RS09180	1915437..1915610	+	174	WP_010971273.1	hypothetical protein	-
F3P66_RS09185	1915689..1917077	-	1389	WP_169539085.1	MFS transporter	-
F3P66_RS09190	1917256..1917504	+	249	WP_006311245.1	hypothetical protein	-
F3P66_RS09195	1917512..1917871	-	360	WP_006311244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F3P66_RS09200	1917871..1918137	-	267	WP_010971270.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F3P66_RS09205	1918207..1918407	-	201	WP_006311242.1	heavy-metal-associated domain-containing protein	-
F3P66_RS09210	1918460..1918882	-	423	WP_006311241.1	Cu(I)-responsive transcriptional regulator	-
F3P66_RS09215	1918879..1921464	-	2586	WP_035256421.1	heavy metal translocating P-type ATPase	-
F3P66_RS09220	1921640..1922248	-	609	WP_010971267.1	class I SAM-dependent methyltransferase	-
F3P66_RS09225	1922356..1922631	+	276	WP_010971266.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
F3P66_RS09230	1922628..1922963	+	336	WP_010971265.1	type II toxin-antitoxin system YafQ family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

• SpoVT-AbrB domain (14-52)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12857.04 Da
• Isoelectric Point: 8.2782

>T139680 WP_006311244.1 NZ_CP043962:c1917871-1917512 [Agrobacterium fabrum]
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA

Nucleotide

• Length: 360 bp

>T139680 NZ_CP043962:c1917871-1917512 [Agrobacterium fabrum]
ATGGTCCGCAACCAGATCCCCAAGCGCGGCGACGTTTATCTGGTTGATCTAAACCCTGTCGTAGGCAGCGAAATCAAGGA
CGAACATCGCTGTGTCGTCATCACGCCCAGAGAAATTAACGCGGTCGGACTCTGTCTCGTCGTCCCGGTGACCACCGGCG
GCATGTTTACGCGCAAGGCAGGGCTTGCCGTAAATATATCCGGCCACAAGACAACGGGCGTCGCTTTGTGCAATCAGGTG
AGAAGCATGGATATCGTCGCCCGGGTTGCCCAGAAGAAAGCGAAATATATCGAAACCCTCGATGATGCGACGATCGATGA
AATCGCCGGGCGCGTCATCAGCATGATCGATCCAGCTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9423.02 Da
• Isoelectric Point: 5.1106

>AT139680 WP_010971270.1 NZ_CP043962:c1918137-1917871 [Agrobacterium fabrum]
MTVTTKIRRQGGAAVMTIPPALLKMLGLEIGEQLTLEVDNGALVASPVRLEKKRFTLAELLDGAEEVAALNARERAWDTA
PPVGKEAL

Nucleotide

• Length: 267 bp

>AT139680 NZ_CP043962:c1918137-1917871 [Agrobacterium fabrum]
ATGACCGTGACCACGAAAATAAGACGCCAGGGCGGCGCGGCGGTGATGACGATCCCGCCCGCTCTTTTGAAGATGCTGGG
GCTGGAAATCGGTGAGCAACTGACGCTCGAGGTCGATAATGGCGCTCTGGTGGCAAGCCCGGTTCGACTGGAAAAGAAAC
GCTTCACGCTGGCCGAATTACTGGATGGTGCAGAGGAAGTGGCAGCTCTAAACGCCAGGGAGCGGGCGTGGGACACAGCG
CCTCCTGTCGGCAAAGAAGCGTTGTGA

Structures

Toxin

• AlphaFold DB: Q7D0B1

Antitoxin

• AlphaFold DB: Q7D0B2