139672

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 147615..148150 Replicon plasmid pST95-32-2
Accession NZ_CP043952
Organism Escherichia coli strain ST95-32

Toxin (Protein)


Gene name relE Uniprot ID V0VJJ7
Locus tag F3J96_RS27420 Protein ID WP_000222760.1
Coordinates 147863..148150 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag F3J96_RS27415 Protein ID WP_001132900.1
Coordinates 147615..147866 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3J96_RS27370 142742..143302 + 561 WP_000139315.1 fertility inhibition protein FinO -
F3J96_RS27375 143438..143650 + 213 WP_001299730.1 ANR family transcriptional regulator -
F3J96_RS27940 143896..143970 + 75 Protein_137 endonuclease -
- 144226..144287 - 62 NuclAT_1 - -
- 144226..144287 - 62 NuclAT_1 - -
- 144226..144287 - 62 NuclAT_1 - -
- 144226..144287 - 62 NuclAT_1 - -
F3J96_RS27385 144274..144480 + 207 WP_001351576.1 type I toxin-antitoxin system Hok family toxin -
F3J96_RS27390 144764..145021 + 258 WP_000083845.1 replication regulatory protein RepA -
F3J96_RS27400 145253..145327 + 75 WP_001442103.1 RepA leader peptide Tap -
F3J96_RS27405 145320..145802 + 483 WP_001273588.1 hypothetical protein -
F3J96_RS27410 145795..146652 + 858 WP_000774297.1 incFII family plasmid replication initiator RepA -
F3J96_RS27415 147615..147866 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
F3J96_RS27420 147863..148150 + 288 WP_000222760.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F3J96_RS27425 148254..148574 + 321 Protein_145 serine acetyltransferase -
F3J96_RS27430 148590..148887 - 298 Protein_146 IS1 family transposase -
F3J96_RS27435 148976..149998 + 1023 WP_000255956.1 IS21-like element IS100 family transposase -
F3J96_RS27440 149995..150777 + 783 WP_001442137.1 IS21-like element IS100kyp family helper ATPase IstB -
F3J96_RS27445 150832..151196 - 365 Protein_149 IS1 family transposase -
F3J96_RS27450 151296..152517 + 1222 Protein_150 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..189994 189994


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11105.15 Da        Isoelectric Point: 10.5832

>T139672 WP_000222760.1 NZ_CP043952:147863-148150 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T139672 NZ_CP058772:107511-107660 [Shigella flexneri]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT139672 WP_001132900.1 NZ_CP043952:147615-147866 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT139672 NZ_CP058772:c107467-107406 [Shigella flexneri]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VJJ7

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References