Detailed information of TA system TA139671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 134977..135602
• Replicon: plasmid pST95-32-2
• Accession: NZ_CP043952
• Organism: Escherichia coli strain ST95-32

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F3J96_RS27345
• Protein ID: WP_000911333.1
• Coordinates: 134977..135375 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: F3J96_RS27350
• Protein ID: WP_000450520.1
• Coordinates: 135375..135602 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3J96_RS27325	131308..131817	+	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-
F3J96_RS27330	131831..132562	+	732	WP_000850416.1	complement resistance protein TraT	-
F3J96_RS27340	132815..134968	+	2154	WP_039025869.1	type IV conjugative transfer system coupling protein TraD	-
F3J96_RS27345	134977..135375	-	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F3J96_RS27350	135375..135602	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..189994	189994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T139671 WP_000911333.1 NZ_CP043952:c135375-134977 [Escherichia coli]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T139671 NZ_CP058772:82470-82775 [Shigella flexneri]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT139671 WP_000450520.1 NZ_CP043952:c135602-135375 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT139671 NZ_CP058772:82250-82468 [Shigella flexneri]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2